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Environmental DNA extractions - (Sep/16/2011 )


can anybody advise any alterations to a normal CTAB DNA extraction, that is more sensitive to environmental anammox bacteria DNA?



there are some sort of Nano Magnetic DNA extraction kits out there, but I think CTAB is more friendly for our environment.


Hi try a ctab/polyvinylpyrrolidone extraction, the PVPP is excellent at mopping up polyphenols and carbohydrates from environmental samples which can be inhibitory to enzymatic reactions