We need a way to see microRNA and DNA in vitro for reverse aging - (Apr/16/2017 )

Imagine being able to observe microRNA and DNA action in vitro, that, with the help of computer visualization, could help decode every mechanics in the body and eventually a way to reverse aging. From 20 second to 40 second of the video about attosecond laser presented.

So how exactly do we reverse aging?

First we need to understand the mechanics, we get a snapshot of the complete DNA genome(gene expression) of every cell in the body in the 30 years old and attempt to reverse every cell's DNA gene expression in the body to match it as we grow older, like when I become 50 years old. We do not need to modify the DNA, just the gene expression. This SALK article shows that it is possible to change the epigenetic marks(gene expression) by expressing the four factors in the cell for two days and have the cell's age slowly reverse back to a younger age, that is before it becomes a stem cell completely. The transgenic mice is modified to respond to an antibiotic that would turn on the four genes, but we cannot do that to our bodies, so we need the help from microRNA to change the gene expression. Now this article shows that it is possible to create induced pluripotent stem cell from microRNA, but as far as I've looked everywhere there really is not an article about microRNA changing gene expression, it's more of a myth how silencing mRNA with microRNA would results lead to a change in gene expression. Hormone changes gene expression, but for microRNA, it's a myth. Now this article suggests a transfection method to get microRNA into the cell using a virus, but I prefer the natural way in which microRNA normally enters a cell, this is yet another problem scientists do not understand, maybe with the help of attosecond laser we can understand the mechanics better.

So the goal is to inject an artificial microRNA that would revert all 50 years old cells' gene expression to match the 30 years old cells' gene expression, but how are we going to test it? My idea is through computer programming, microRNA are codes and it binds to mRNA to silence it and eventually leads to a change in gene expression, such calculations can be predicted with computer computations. I'll leave the rest to your thoughts an feedback. Thanks for taking the time to read this

Light sheet microscopy is on its way to visualize cell in vivo, correction, in vivo, here, at 250nm resolution

"I've looked everywhere there really is not an article about microRNA changing gene expression, it's more of a myth how silencing mRNA with microRNA would results lead to a change in gene expression"  There is an extensive literature on this topic.  Have you tried PubMed at NCBI? ncbi.nlm.nih.gov

Jon Moulton on Wed May 24 18:51:34 2017 said:

"I've looked everywhere there really is not an article about microRNA changing gene expression, it's more of a myth how silencing mRNA with microRNA would results lead to a change in gene expression"  There is an extensive literature on this topic.  Have you tried PubMed at NCBI? ncbi.nlm.nih.gov

Well, I know how hormone regulates gene expression by phosphorylating transcription factors and have these transcription factors enter the nucleus to change the gene expression. But how does microRNA changes gene expression if all it does is silencing mRNA? And how does silencing microRNA activates transcription factors? I might have missed something here, but do enlighten me if you know the answer :D

So now you can see microRNA at work. But it would not be of much help unless you know where it came from or in which tissue this microRNA is produced. If you can capture in molecular detail of the full body in 3D then we can trace and track each of the microRNA and cell like a movie. Of course this requires visualization technique to rule out the structures possibly with a database, identify and correlate based on similar findings of the molecules and essentially the entire body.

We figure it will be easier to test microRNA with a computer simulation then on live animals. We can test a variable microRNA without having to inject it into a live specimen each time.

P.S Work with dream groups, they are more experienced in scanning

Reverse aging would be done either through regeneration or wound healing

"Well, I know how hormone regulates gene expression by phosphorylating transcription factors and have these transcription factors enter the nucleus to change the gene expression. But how does microRNA changes gene expression if all it does is silencing mRNA? And how does silencing microRNA activates transcription factors? I might have missed something here, but do enlighten me if you know the answer "

miRNAs bound within RISC bind to partially-complementary sites called micro-RNA response elements (MRE) usually located in the 3'-UTR of mRNAs.  With the RNAs looped into circles for translation, this brings the 3'-UTR-bound RISC close in space to the cap on the 5'-UTR, where the initiation complex forms for cap-dependent translation.  The presence of the RISC near the initiation complex represses translation of the mRNA. To repress the translation of an mRNA is to change gene expression.  Silencing = change in expression.

As for activation of transcription factors, there is complex logic in gene expression and the ability to repress expression at one point can lead to many different outcomes.  For instance, if expression of a transcript factor is repressed by a protein that binds DNA at the operator of the transcript factor and if the mRNA for the repressor protein contains an MRE, then binding an miRNA to that MRE would suppress translation of the repressor's RNA and so the transcription factor expression would increase as the level of translated repressor protein decreases.

Thanks for the explanation. Injecting miRNA into the body to reverse cells into a younger state seems possible with the partial reprogramming method without going back to stem cell. But if you want to reverse age back to a younger body type like 5 years old this is where it seems impossible. If you compare this with immortal jellyfish it seems the outer shell slowly deteriorates. It's not like you puncture a balloon and it becomes smaller, it is like you are blowing a balloon inside a balloon. I don't think there is an elegant solution for this. Someone enlighten me how biological immortality would work. Why going back to 5 when you can stay 30, well you would lose teeth and the only way to regrow them is by going to a younger age. If staying 30 is your solution the SALK method seems possible, human trial in 10 years.

This seems like a promising research about regeneration from a different approach. First, regenerate a younger limb, then an entire body

Well, we'll have to prove that bioelectricity is capable of inducing cell's reverse aging though, if Jon Moulton has an answer do let me know sir. This chart, in reverse, is the answer to reverse aging. Right to left.