DNA extraction , phenol , ice . plz help ! - (Oct/13/2013 )
hi every one , i wanna know why we put our solution on ice after we add phenol to it .
any help is appreciated
As far as I know there isn't any need to ice during the phenol step (unless perhaps you are working on something with a lot of DNases). I have done many a DNA phenol extraction where the only time I would use ice is during the DNA precipitation.
You do not need to keep on ice you get better separation at RT. Even for precipitation RT works better.
my supervisor told me so