I dissolved the DNA in 1XTAE instead of TE buffer, will it affect ligation? - (Oct/03/2013 )
After dephosphorylating the vector, I extracted it with phenol/chloroform extraction and ethanol precipitation. However, in the last step after rinsing the DNA with 70% ethanol and drying the DNA, I accidentally dissolved the DNA with 1XTAE buffer instead of TE buffer. Now I want to use this DNA solution for ligation. Will it work? I am just adding 1ul of this vector solution to a 10ul ligation reaction
You should be fine. I once used a sigma kit and they gave you an alternative ligation buffer if you suspended your DNA in TAE. When you are finished with your ligation, I would consider purifying your product somehow. TAE has been shown to significantly decrease the transformation efficiency of E. coli.
Google search pulled up the relevant kit with alternative buffer: