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Will long exposures of DNA to UV (302nm) alter sequencing data? - (Aug/19/2013 )

dideoxynucleotide based sequencing, to be exact?

-Ahrenhase-

I would think so, yea.  UV (UV-B, to be precise, at 302nm) causes dimers between C's and T's.   This, and also the fact that dimers will change the DNA's shape, might interfere with the sequencing reaction.

-Glynn-

Sequencing results will probably not be affected, although the quality of the sequencing will likely be impacted. It's hard to imagine a way in which you would get a different sequence from UV exposure.

-phage434-

Sequencing results will probably not be affected, although the quality of the sequencing will likely be impacted. It's hard to imagine a way in which you would get a different sequence from UV exposure.

Wouldn't a polymerase pop off at the first thymine dimer?

-Ahrenhase-

Perhaps a single polymerase at a single location, but remember that there are billions of such polymerases, all active at the same location at the same time. When a single or few of those fall off, nothing will happen to the sequence read.

-phage434-