DNA fragmentation - (Jun/20/2012 )
Hi to All
I am trying to assess apoptosis induction by DNA fragmentation (DNA laddering).
I lysed my cells by lysis buffer and purified DNA by Phenol-chloroform- isoamylalcohol. But unfortunately I have'nt detect the ladder until now. I seed the cells in 6 well duplicate for each concentration. and I run 6microgram DNA in agarose gel .
I would be glad and appreciated If anybody introduce a sufficient protocol.
Have you tried everything in this protocol?