I am trying to extract DNA bands from 4% acrylamide gel, but I had no success. Does anybody thinks that the age of acrylamide gel (my gel stock solution is about a year old) can affect the extraction of DNA bands from the gel.
-saara-
In my experience, the acrylamide (if stored at 4C) should be fine. We also never "extracted" DNA from the gels, just cut the bands and placed them into 10mM Tris for 24-48hrs in the fridge. Over that time most of the DNA escaped from the gel into the liquid...at least enough for PCR. Hope that helps.
saara on Nov 1 2009, 02:23 PM said:
I am trying to extract DNA bands from 4% acrylamide gel, but I had no success. Does anybody thinks that the age of acrylamide gel (my gel stock solution is about a year old) can affect the extraction of DNA bands from the gel.
-ShannonJ-
ShannonJ on Nov 2 2009, 04:01 PM said:
In my experience, the acrylamide (if stored at 4C) should be fine. We also never "extracted" DNA from the gels, just cut the bands and placed them into 10mM Tris for 24-48hrs in the fridge. Over that time most of the DNA escaped from the gel into the liquid...at least enough for PCR. Hope that helps.
so we don�t need to use the gel extraction kits?
Awesome cost saver.... -Mulletman-
I always have for agarose gels but not for acrylamide...but like I said you had to let 'em sit in the fridge for at least a full day. You might get better recovery with an extraction kit but we were just re-PCRing from DGGE gels and it worked pretty much without fail.
Mulletman on Nov 3 2009, 05:04 AM said:
so we don�t need to use the gel extraction kits?
Awesome cost saver....
-ShannonJ-
