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No band after extracting DNA from bacillus subtilis - (Nov/09/2011 )

Hi, I'm doing Bacillus Subtilis and I'm required to extract the total DNA from it. Is there any efficient extraction method for Bacillus?

I've try phenol chloroform method and the ethanol precipitation. But the amount of DNA extracted is too little that no band is spotted after I run a gel. Need help.....


For Gram +ve bacteria, it is better if you can crush/grind it with liquid nitrogen before you proceed with your PCI method.
What is your downstream application?

-Adrian K-