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What happens to DNA in the presence of a chaotropic salt? - (Nov/17/2012 )

I've used 4M Guanidine Hydro Chloride (GuHCl) for Plant RNA Viroid extraction with 25mM EDTA, 0.2M Sodium Acetate (NaAc) (pH 5), 2.5% PVP 40. Could you please tell me ;

How RNA get separated from DNA when using GuHCl?

What is the purpose of adding NaAc in Nucleic Acid Extraction?

-Akila Wijerathna Yapa-

What is the purpose of adding NaAc in Nucleic Acid Extraction?
http://www.protocol-online.org/biology-forums/posts/3846.html

-memari-

Guanidine is a chaotrope, so it essentially denatures the proteins so that they won't stick to the DNA.

-bob1-

Thank you very much memari & bob1

-Akila Wijerathna Yapa-