DNA extraction from paraffin embbed tissue - How can extract DNA form tissue (Nov/11/2010 )
I want to extract DNA from paraffin embbeding tissue which already stain with H&E. After staining, i have to select cell from tissue by manual technique. However, i don't know what is the buffer that use to pick up cell from tissue (Should have protease in buffer? ). What is chemical component of that buffer? How i get DNA from that tissue which have very low amount of cell by use phenol chloroform technique?
Note: My tissue have 4 um thick and have area 1x1 cm (liver section)
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Thank you so much.