Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

DNA extraction from paraffin embbed tissue - How can extract DNA form tissue (Nov/11/2010 )

Dear all

I want to extract DNA from paraffin embbeding tissue which already stain with H&E. After staining, i have to select cell from tissue by manual technique. However, i don't know what is the buffer that use to pick up cell from tissue (Should have protease in buffer? ). What is chemical component of that buffer? How i get DNA from that tissue which have very low amount of cell by use phenol chloroform technique?
Note: My tissue have 4 um thick and have area 1x1 cm (liver section)

Thanks

-Parichut-

Hello Parichut,

have a look at this Protocol Online Archives
i remember discussing this here, before ...

hope you will find it beneficial :)

Best Wishes

-nightingale-

Thank you so much. :lol:

-Parichut-