How clean do I need my DNA to be? - (May/18/2017 )
I have been using kits with columns to purify genomic DNA which I then use as a template for PCR for sequencing or T7E1/CelI mutation-detection assays. I accidentally ordered a kit which does not use columns but rather the older approach of chloroform and ethanol precipitation. I think it should be fine but then I wonder why I've been using spin columns... just for convenience?
-seanspotatobusiness-
Partly correct, convenience is one of the features; they also save time, which, if you are employed, is frequently the biggest cost for any experimental laboratory.
-bob1-