Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Cloning

partial DNA digestion formula - Linear DNA partial digestion (Mar/08/2010 )

Hello ,
A linear DNA fragment ia 100% labeled at one end and has 3 restriction sites for E.co R1 .If it is partially digested by EcoR1 so that all fragments are produced , how many of these fragments will be labeled and how many will nt be labeled?

What will happen if the DNA is completely digested ?

-pallabi-

Hello pallabi, welcome to the BioForum!

This sounds like a homework question -- is it? If so, it belongs in the Homework Questions thread, where members will help you arrive at the correct answer, but won't just tell you the answer -- you have to show us what you've tried on your own to arrive at the answer.

Let me know and I'll move your question if appropriate.

-HomeBrew-