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RNAse treatment of genomic DNA - concentration and duration? - (Oct/04/2011 )

Hello everyone,

I need RNA-free genomic DNA for my qPCR. For DNA isolation I have a kit indicating I can do RNAse treatment, but it does not indicate at which concentration and how long.

So maybe there is someone with experience about that? Thanks a lot for any help!

:o)

-greenhorn-

1 ug/ml for 20 min at 37 should do it.

-bob1-