RNAse treatment of genomic DNA - concentration and duration? - (Oct/04/2011 )
I need RNA-free genomic DNA for my qPCR. For DNA isolation I have a kit indicating I can do RNAse treatment, but it does not indicate at which concentration and how long.
So maybe there is someone with experience about that? Thanks a lot for any help!
1 ug/ml for 20 min at 37 should do it.