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Isolation of DNA from Scat - (Mar/04/2010 )

Hi
I am trying to isolate the DNA from scats of vertebrates. I tried Phenol:Choloroform & GuSCN methods. but i am not able to get desired DNA. If I got somethings its not amplify in PCR properly and also some bacterial DNA is also there. So plz tell me how can i got the desired bateria free DNA from Scat

-atulkathait-

We have had good results isolating bacterial DNA for PCR amplification from mouse and human feces using the ExtractMaster fecal DNA extraction kit from
Epicentre Biotechnologies.

-HomeBrew-

Try a Qiagen fecal kit. There's also MoBio kits - they generally will send you a couple preps for free to try them out - and they're good for environmental stuff.

-microgirl-