Protocol Online logo
Top : New Forum Archives (2009-): : Electrophoresis

syto9 for DNA gel electrophoresis - (Sep/20/2011 )

Hi there
has anyone tried using syto9 to stain for DNA in gels?
ie, mix some syto 9 to your PCR rxn, incubate for 5 mins, load on to gel


Does it actually bind to the DNA or is it intercalated (similar to EtBr and Sybr)? If it actually binds, it should be OK; otherwise you would need to check whether it will migrate in the same direction as the DNA on a gel.