What is the expected pattern of restriction digestion of human genomic DNA? - (Jun/17/2011 )
I am trying to do long distance inverse PCR (LDI-PCR), which requires restriction digestion of genomic DNA. I tried digesting my genomic DNA with BglII, XbaI and BamH1. I have the picture of the agarose gel attached to show you how it looks like after 2 hours digestion. gDNA1-4 are from the same cell type just different way of DNA isolation. Could anybody tell me if my digestion worked? Or what a good digestion pattern should look like? Thanks so much!
The picture didn't come through, but you should expect a smear, centered around about 4 Kb, the expected length of random DNA sequences cut with a six base cutter.
Thank you so much, Veteran!