RNA contamination in DNA samples - (Aug/08/2013 )
I would like to know if a DNA sample with RNA contamination can be used for transfecting B16, 293H and SH SY5Y cells.
Thanks for the help!
Is the DNA sample plasmid? I don't think that will be a problem.
Yes, it is!
I thought that only protein/lipids contamination could interfere in this method, but my transfection (using the RNA contaminated DNA sample) didn't work . In parallel, I transfected B16 cells with another plasmid with a EGFP tag (without contamination) and it worked well. And to make things worse, when I used both plasmids, neither the EGFP nor the RNA contaminated worked.
Maybe I could try a RNAse treatment (again) following by phenol/chlorof extration and ethanol precipitation in order to purify my DNA (because I usually do home made Midiprep Oh God, I need thoseMidi/Maxiprep Kits!!)... What do you think about it?