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Seperate DNA spin columns - (Feb/17/2009 )

I am currently assessing cell lysis techniques that would be most suitable for my research. To do this I want to test lysis separately from purification. Because of this I need a standard DNA purification method which I can use when I change the lysis method I am testing.

I would like to use a DNA spin column as my purification standard. However so far I have had no luck finding any companies that sell the DNA spin columns separate from the lysis buffers in their kits.

Does anyone know where I can buy spin kits seperately? The only ones I have found so far have either been for RNA, to clean up PCR products or to extract DNA from agarose.

-D I Walker-

D I Walker on Feb 17 2009, 07:36 AM said:

I am currently assessing cell lysis techniques that would be most suitable for my research. To do this I want to test lysis separately from purification. Because of this I need a standard DNA purification method which I can use when I change the lysis method I am testing.

I would like to use a DNA spin column as my purification standard. However so far I have had no luck finding any companies that sell the DNA spin columns separate from the lysis buffers in their kits.

Does anyone know where I can buy spin kits seperately? The only ones I have found so far have either been for RNA, to clean up PCR products or to extract DNA from agarose.


MOBIO (http://www.mobio.com/index.php) sells component separately from their kits. When you go to their website and look at their kits....go to the lower bottom right corner. There are the components of the kit, which most of the time can be purchased separately. For example, here are the kit components for the UltraClean DNA isolation kit:

http://www.mobio.com/products/productdetail.php?pid=90

Hope that helps.....Cheers!

-Wolverena-

Thanks for your reply. I have also found another company that sells spin columns seperately.

PeqLab (www.peqlab.co.uk)

-D I Walker-

I think promega sells it as well.

-T C-