PROTOCOL FOR LINEARIZED DNA - (Nov/26/2015 )
I want to linearize my plasmid so i will be able to transfect it
The enzyme that i will use is Notl
anyone have experience with such protocol?
Basically mix the DNA with the appropriate restriction buffer and enzyme in a final volume of 50 ul. Digest for 1 hour at 37. Check digest has worked by running on gel. Purify DNA and use for transfection.