DNA dilution 1, 2, 4, 8, 10 X - (Jun/14/2015 )

Hi there,

 I am new to lab techniques. I need to dilute my DNA to 1, 2, 4, 8, 10 X. I have total volume of 500ul and 5ng/ul.

Would be great if someone can explain how I should dilute it. The volume I would be needed 10 ul. 

Thanks in advance, 

The dilution series is wrong. It should be 1:2, 1:4, 1:8, 1:16, 1:32 and 1:64

1:2 dilution means 1 part of substance : 2 part total.

Now, use the following formulae and calculate:

Move Volume = Final Volume / (DF -1)

Diluent Volume = Final Volume – Move Volume

Total Mixing Volume = Diluent Volume + Move Volume