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Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood - (Feb/27/2013 )

Hi All,

I want to do DNA isolation form whole blood and I need to make these buffers ... TKM1 and TKM2

Can anyone help me with the recipe for the TKM1 and TKM2 buffers.

Thank you


TKM :10mM Tris-HCl, pH 7.6, 10 mM KC1, 2 mM EDTA (TKE) containing 4 mM MgCI2 (TKM). No idea about the second one though. It will be a high salt buffer.