Precipitation in DNAse I incubation buffer - (Apr/19/2010 )
I have a problem with the buffer of DNase I from Roche. I detected a white precipitate in the buffer. Here's the recipe for the buffer:
Incubation Buffer (10�)
400 mM Tris-HCl, 100 mM NaCl, 60 mM MgCl2, 10 mM CaCl2, pH 7.9.
Some lab partners said that sometimes that happens to the incubation buffers, and that it could be remediated with a few minutes at 37�C, but in this case that wasn't the solution. Does someone knows what it's going on? can be remediates or it's better to throw it away?
-vabarca-
vabarca on Apr 19 2010, 05:15 PM said:
I have a problem with the buffer of DNase I from Roche. I detected a white precipitate in the buffer. Here's the recipe for the buffer:
Incubation Buffer (10�)
400 mM Tris-HCl, 100 mM NaCl, 60 mM MgCl2, 10 mM CaCl2, pH 7.9.
Some lab partners said that sometimes that happens to the incubation buffers, and that it could be remediated with a few minutes at 37�C, but in this case that wasn't the solution. Does someone knows what it's going on? can be remediates or it's better to throw it away?
Incubation Buffer (10�)
400 mM Tris-HCl, 100 mM NaCl, 60 mM MgCl2, 10 mM CaCl2, pH 7.9.
Some lab partners said that sometimes that happens to the incubation buffers, and that it could be remediated with a few minutes at 37�C, but in this case that wasn't the solution. Does someone knows what it's going on? can be remediates or it's better to throw it away?
I would throw it away. It's not worth it to troubleshoot and find a remedy for a buffer that can be easily re-made.
-ProteinWork-