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Precipitation in DNAse I incubation buffer - (Apr/19/2010 )

I have a problem with the buffer of DNase I from Roche. I detected a white precipitate in the buffer. Here's the recipe for the buffer:

Incubation Buffer (10×)
400 mM Tris-HCl, 100 mM NaCl, 60 mM MgCl2, 10 mM CaCl2, pH 7.9.

Some lab partners said that sometimes that happens to the incubation buffers, and that it could be remediated with a few minutes at 37ēC, but in this case that wasn't the solution. Does someone knows what it's going on? can be remediates or it's better to throw it away?

-vabarca-

vabarca on Apr 19 2010, 05:15 PM said:

I have a problem with the buffer of DNase I from Roche. I detected a white precipitate in the buffer. Here's the recipe for the buffer:

Incubation Buffer (10×)
400 mM Tris-HCl, 100 mM NaCl, 60 mM MgCl2, 10 mM CaCl2, pH 7.9.

Some lab partners said that sometimes that happens to the incubation buffers, and that it could be remediated with a few minutes at 37ēC, but in this case that wasn't the solution. Does someone knows what it's going on? can be remediates or it's better to throw it away?

I would throw it away. It's not worth it to troubleshoot and find a remedy for a buffer that can be easily re-made.

-ProteinWork-