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Labeling DNA with Biotin - (Sep/23/2013 )



I would like to label some DNA wth Biotin by PCR. I can buy two kind of Biotin labeled dUTP nucleotides: Bio-11-dUTP and Bio-16-dUTP. Which one do I need?






Either is fine. The -16 has a slightly longer linker, which may make it a bit easier to bind in some downstream applications. It probably does not matter.

Don't forget you need to use Taq or a Taq + Pfu like mixture for PCR to incorporate the dUTP.