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Can we use denatured DNA standards on RNA formamide gels - (Feb/23/2012 )


Is it possible to use denatured DNA standards on RNA denaturing gels in order to evaluate the size of RNA. I've used denatured DNA in order to evaluate some known mRNA on northern blot and it gave correct size but I couldn't find any literature saying whether you could use DNA standards or otherwise. Hence, I would be much grateful if someone enlightens me regarding this topic.

Please find the attached copy of the blot.

Thanking you.
Attached Image


In theory it should work, though there might be slight charge differences that could change the apparent size of the product.


and keep in mind that you are no longer looking at "base pairs", you are looking at "bases".