Qiagen DNAeasy blood&tissue DNA isolation kit - (Jul/29/2009 )
Is there anyone use Qiagen DNAeasy blood&tissue DNA isolation kit? I was useing this kit to isolate DNA from the tail of mice for genotyping. I had a lot of problem with the genotyping PCR these days. I changed the PCR reagents, but still don't work. Then I measured the DNA concentration and found that the concentration is as low as 20ng per microlitle.
I am asking if anyone here had the same bad experience and any of your suggestion will be appreciated.
How old is the kit? Are any of the buffers out of date / stored at the wrong temperature / precipitated?
Also I find the recommended final elution step can be too high, I only use 30ul as a standard