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Tips for genomic DNA extraction from gram -ve bacteria - (Feb/23/2009 )

I am new to genomic DNA extraction and seeking advises. Are there any tips? I am rather unsure about removing protein by phenol:chloroform, how much volume (e.g v/v ratio?) should I add into the lysate?

-Nrelo-

Nrelo on Feb 23 2009, 11:02 PM said:

I am new to genomic DNA extraction and seeking advises. Are there any tips? I am rather unsure about removing protein by phenol:chloroform, how much volume (e.g v/v ratio?) should I add into the lysate?



1:1

-hanming86-

You really don't need to use phenol/chloroform these days. Gram positive bacteria are tough to lyse, but with a combination of lysozyme, beadbeater, and sonicator to break up the cell walls in AquaGenomic or AquaRNA solution you will get the DNA you need.

-AquaPlasmid-