Problem with gel extraction of DNA. - (Aug/28/2010 )
I was doing gel extraction of my DNA today and after cutting the band portion, I mistakenly dropped it on floor. I know it is foolish and carelessness. Anyways, I still proceeded to extract the DNA. In the mini column which I was using for clean up, I found little dust particles. Do you think that there might be some contamination in my DNA?
It could be.. but since you allready did the extraction and proceeded.. you can continue and see what happens. If it wont work.. well too bad, if it does work...
I once had a similar problem: dropped a part of some DNA bands on the table, just used it as the others and didnt see any difference in the end..
I however did an extra cleaning step (I do it always): ethanonpurification.