Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

My DNA pellet doesn't dissolve - Overdried?? (Feb/22/2011 )

Hi.

I did a DNA extraction but now I can't dissolve the pellet in TE. I think I might have overdried the pellet. What can I do? The tubes are currently at 37 C, but no luck until now.


Thanks in advance.
:rolleyes:

-cardosopedro-

keep it at 37C and give it some time

-mdfenko-

Dissolve the DNA overnight on a shaker or at 55C for 12 h.
this was what i did using Qiagen genomic DNA extraction kit.

-nightingale-

Thanks, I will try that right now!

-cardosopedro-