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How many fibroblasts for 1ug DNA? - (Dec/05/2013 )



Does anyone have an estimate, how many primary human fibroblast are needed to isolate 1ug of DNA? How many/which flasks of adherent cells do you normally harvest for 1ug of DNA?

I am normally a suspension-culture person, if anyone knew would be awesome!


Thanks a lot,



According to Roche Lab FAQs, there is about 6 pg of total DNA per cell in a human cell, so I work that out to be about 166,667 cells.


Oh - great! Thank you! :)