DNA isolation from nuclei - (Jul/20/2013 )
Hello! Has anyone ever isolated mammalian DNA from nuclei? I can make nuclei really easily for DNase Hypersensitivity experiments and I receive significant yields at the end, so I thought it should be straightforward to isolate DNA directly from nuclei rather than from a Qiagen kit. Thanks!
not sure what you mean? Nobody forces you to use a qiagen kit... So I dont see what your problem is or what you question is? Do you mean whether you could use another type of DNA extraction system than the qiagen kit?
Thanks for the reply.
Normally, I isolate DNA from 5 million cells or digest tissue overnight, either with a qiagen kit or routine phenol-chloroform/ethanol-ppt. Just curious about whether DNA from nuclei can be isolated using phenol-based method.
hotstuffdb22 on Sat Jul 20 16:41:37 2013 said:
Thanks for the reply.
Normally, I isolate DNA from 5 million cells or digest tissue overnight, either with a qiagen kit or routine phenol-chloroform/ethanol-ppt. Just curious about whether DNA from nuclei can be isolated using phenol-based method.
I am a bit confused. I dont really understand your questions.... You do a DNA extraction with the phenol-based method, so of course you can ge the DNA from the nuclei (where else would the DNA come from?) I am not sure I understand you.... You work with tissue, so I assume its from a eukaryotic organism?
I apologize for the confusion. I'm not being clear here. Thanks for the feedback!
hotstuffdb22 on Sun Jul 21 20:48:25 2013 said:
I apologize for the confusion. I'm not being clear here. Thanks for the feedback!