Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

Viral DNA extraction - (Oct/12/2010 )

Hi bioforumers
Iam startig to wrk in a new project,my first task is to do PCR from lyophilized varicella vaccine (Oka strain)
Should I do direct PCR or DNA extraction and which protocol should I use

-desertrose-

DNA extraction should give better (as in more consistent) results. A standard phenol-chloroform preparation should work well.

-bob1-