DNA purification - DNA purification dissolved in water (Apr/19/2010 )

Hi,

I have measured the DNA concentration and the optical density is quite low (~1,6). The DNA is dissolved in water. Is it possible to precipitate it with isopropanol, spin it down and wash it with ethanol? Is there a better way to do that?

mic on Apr 19 2010, 02:10 AM said:

if you have added only 30ul of water in the end of purification step then just top up your sample with 1.5 ml IsoP, incubate at RT for 5 min and centrifuge at 13000 RPM for 10-15 min.

Why do you want to try other methods? this is the easiest and fastest way. IsoP and ethanol are available in any lab.

Quite low for what? That is a very reasonable concentration of DNA. You can precipitate and wash, but will lose some DNA.

mic on Apr 19 2010, 06:10 AM said:

OD 1.6 equals roughly 80ng/ul for double-stranded DNA. That's not low at all. It might not be the highest yield for a miniprep, but definitely within the normal range I've seen. For most applications I know, that concentration is more than enough.