Extraction of large size genomic DNA - (Apr/16/2009 )
I want to extract genomic DNA which is up to several mega base pair from Burkholderia, are there any commercial kits capble to do so? Thanks
What do you want to do with this DNA. If you need it in whole chromosomes, then you need to embed cells in agarose and digest them in-place. If you just need DNA for sequencing, library construction, or PCR, then any normal genomic prep will work fine. I've had a lot of success with the Zymed 96 well plate format preps, but also with the Sambrook CTAB protocol.
phage434 on Apr 16 2009, 04:08 AM said:
I have inserted a gene into the bacterial genome and want to find out the insertion site. So I need to extract the genomic DNA, digest it with a R.E, ligate the fragments and sequence them. In this case, any normal genomic prep will work?