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Ethanol precipitation of DNA. How long...? - (Oct/30/2012 )

Hi everyone!

I've a question about ethanol precipitation of DNA.

Does anyone know how long can I leave the DNA precipitating at -80º?

Do you think a weekend is too much (well, it's too much, I normally performe this step in 20 minutes but...) ? I don't think that it will affect DNA integrity, but have no proof. If anyone has any idea it will help a lot!

Thanks in advance!


Indefinitely. It's a good way to store DNA for really long times. This is a common way of storing RNA to limit RNAse activity.


about 2 hours or longer


Thanks a lot! It really helps me out!