Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Cloning

DNA ligation - (Oct/27/2009 )

i use this PCR cloning kit to ligate my DNA. in the handbook it says like this:

DNA product 4 microlitres
Vector 1 microlitres
master mix 5 microlitres

but i have modified the amount of DNA product and the vector, where the amount is:
DNA product 2 microlitres
Vector 7 microlitres
master mix ?

How much of master mix should i added in my modification in order to get a successful transformation and it is possible? tq


really? are you asking how to do a 1x buffer from a 2x buffer? or maybe I did not understand you...


opps. thanks for replying me. here is the new one