can we extract DNA from snap freeze tissue - (Aug/21/2012 )
Dear all,
Can we still able to extract DNA from snap freeze tissues (lungs, pancreas) of mice {snap freeze was done by using pentane in liquid nitrogen}?
May i know the best conventional method to extract DNA from these frozen tissues?
Thank you very much
wdyeo
-wdyeo-
Yes you can.
Any one of the conventional methods will work well, it all depends on the purity you require. For most applications a standard phenol-chlorofom extraction is good.
-bob1-
Dear bob1,
Thanks alot for the reply
Have a nice day
-wdyeo-