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can we extract DNA from snap freeze tissue - (Aug/21/2012 )

Dear all,

Can we still able to extract DNA from snap freeze tissues (lungs, pancreas) of mice {snap freeze was done by using pentane in liquid nitrogen}?
May i know the best conventional method to extract DNA from these frozen tissues?
Thank you very much

wdyeo

-wdyeo-

Yes you can.

Any one of the conventional methods will work well, it all depends on the purity you require. For most applications a standard phenol-chlorofom extraction is good.

-bob1-

Dear bob1,
Thanks alot for the reply
Have a nice day :D

-wdyeo-