Viral genomic DNA isolation ? - (Nov/21/2012 )
Hi, I am very new to virus field. I want to isolate genomic DNA of virus
My friends told me there re 2 typed of protocols - 1. Involves cell lysis
2. Directly pellet the cultured cells and use for DNA isolation
I have genomic isolation kit for bacteria. Is it necessary for me to order new kit for Virus DNA isolation ?
Which one of the above protocol should be used ? I need the Genomic DNA for PCR ?
Thank you for your time.
I know Qiagen has a kit or protocol for genomic DNA extraction of this virus. Check their site.
You can use either.
Are you isolating from tissue culture supernatant? Or a sample of some kind?
I probably wouldn't shell out for a kit if you are using it for PCR, unless you think you'll need really pure DNA.
Pardon me - but I am new to this field
Yes the virus will be from tissue culture. To recover virus
But he also showed me another protocol without any cell lysis. so I am confused what I should use ?
Yes as leelee suggested - PCR doesnt need pure DNA - if there are any other methods please suggest
I want to sequence this PCR before cloning so I am thinking if the DNA is pure it would be better in PCR.