Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Cloning

PhiX174 DNA - (Oct/21/2012 )

Hi I have one doubt in Bacteriophage PhiX174 .. I will transform PhiX174 Dna into E. coli cells. after transformation we can isolate colonies for use any antibiotic resistance or antibiotic free medium?
Thank u in advance.

-biovinz-

PhiX174 won't have native antibiotic resistance so presumably you are cloning some of it into a plasmid - how would you then select for the plasmid?

Note: I think this is a homework question, so I'm making you think a bit...

-bob1-