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internal control for genomic DNA - (Jul/29/2010 )

Hi all,

I would really appreciate some suggestions of internal controls I can use in my PCR using genomic DNA as a template.
I am working with human blood or marrow samples looking for chromosomal rearrangements. Is it possible to quantify rearrangement wrto a some sort of housekeeping gene when working with gNDA?

Thanks very much

-labscientist-

For the expensive solutions, ABI sells IPC kits if you don't want to make them.
https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=602714

Or you can use the Quantifiler kit that has everything in it
https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=catNavigate2&catID=601641

If you'd rather make your own IPC, just browse the literature. There are plenty published.

Can't respond to the second question, sorry.

-Maddie-