Isobutanol extraction of DNA - (May/31/2010 )
in order to concentrate DNA I need to do an isobutanol extraction followed by a diethyl ether extraction. I'm a little puzzled as to whether I should use BioUltra grade isobutanol that has been tested negative for DNAse/RNase or whether I can use the significantly cheaper isobutanol (what are the odds that DNAse survives isobutanol)?
Does anyone have any ideas on that?
You should use molecular biology grade reagents to enure that you are not contaminating your samples with DNA that may be in lower grade reagents.
Hm, I figured... thanks Bob!