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DNA quantification - (Feb/17/2009 )

Hi

I just received plasmid DNA from another institution which mentions 1.0 γ/λ as its conc. I am not familiar with that terminology. Can anybody help me with this?

Thanks.

-1stmolar-

1stmolar on Feb 17 2009, 12:05 PM said:

Hi

I just received plasmid DNA from another institution which mentions 1.0 γ/λ as its conc. I am not familiar with that terminology. Can anybody help me with this?

Thanks.


"Lambda" and "kappa" are easier to say than "microliter" and "microgram". These abbreviations have been used in labs for many years.

Your plasmid is at 1 g/L.

-klinmed-

That's a gamma, not a kappa.

gamma = 1 microgram (Gram)
lambda = 1 microliter (Liter)

Only the real old timers use these any more. Respect them, they figured it out before you, with much, much worse tools!

-phage434-

Thanks guys.

-1stmolar-

phage434 on Feb 17 2009, 11:45 AM said:

Only the real old timers use these any more. Respect them, they figured it out before you, with much, much worse tools!

talk about the pot calling the kettle....

i still use "lambda", never really used "gamma" but am familiar with the terminology.

old habits...

-mdfenko-

We always write this way on microfuge caps to save some room. Some even just write the numeric number to mean ug/ul. lol.

-AquaPlasmid-