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mitochondrial DNA - (Sep/04/2012 )

HI EVERYBODY
can any one send me a simple protocol to MITOCHONDRIAL DNA extraction ? and how can i confirm after that that my DNA extracted is truly mitochondrial
thaaaanks

-lula-

usually it is extracted together with genomic DNA. As there are many mitochondria in the cells you'll have sufficient in your gDNA finally with all protocols.
If you need pure mtDNA, one way would be ultra-centrifugation to get a fraction of cell organelles with mitochondria and then DNA extraction.

-hobglobin-

BUT WHICH KIT IS BETTER ? OR IS SALTING OUT TECHNIQUE FOR GENOIMIC DNA IS SUFFEICIENT

-lula-

I'd say most kits are reliable (I only used a few and did not find much differences, except the price). Salting out works too for mtDNA but the DNA is more "dirty", which is for PCRs usually no problem.

-hobglobin-

by dirty u mean with organells or genomic DNA?
and how can u measure ur mitochondrial DNA amount ( purre mt DNA not geneomic)?

-lula-

mostly proteins of course...and other stuff depending what source of DNA you have
no idea how you can measure the mtDNA amount

-hobglobin-

thank u for ur replies

-lula-