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Buffer Related Discussions
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Buffers - (reply: 2)
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buffer preparation - (reply: 2)
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Blocking buffer recipe - (reply: 3)
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Washing buffer and sauton medium - Survival assay in poor condition (reply: 3)
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reaction buffer for ABTS - any protocol? (reply: 1)
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asking for kanamycin stock solution... - (reply: 4)
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protein band is shifted after boiling sample with sample buffer - (reply: 7)
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protein band is shifted after boiling sample with sample buffer - (reply: 4)
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MAKING A COLLAGEN SOLUTION - (reply: 1)
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Sample Buffer - (reply: 1)
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buffer preparations - reason (reply: 5)
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Dialysis Buffer for Recombinant Protein - (reply: 5)
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buffers effect on size exclusion chromatography.. - amm bicarb, phospate with or without salt/ethanol. (reply: 2)
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FACS sorting into what solution for RNA isolation? - (reply: 2)
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Anode/Cathode buffer in Tris-Tricine SDS-PAGE - purpose of seperate buffers in anode and cathode (reply: 2)
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PBS and STE Buffer - (reply: 2)
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alternative coating buffers for ELISA - anything else i can use aside from PBS and carbonate/bicarbonate? (reply: 6)
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phosphate buffer - (reply: 2)
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Lysis buffer - (reply: 1)
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RNA gel loading buffer? - (reply: 5)
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PBS buffer with or without KCl? - (reply: 2)
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Tris or Hepes? - buffer choice (reply: 1)
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Cloudy destaining solution...why! - (reply: 5)
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how to prepare 0.1 M sodium acetate buffer, pH 5 - i'm bad with my chemistry! (reply: 2)
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Can phenol be mixed with QG buffer from Qiagen Kit - (reply: 7)
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DMSO in western lysis buffer - (reply: 1)
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EROD Assay procedures and solutions experts please help - (reply: 3)
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treatment buffer concentration - (reply: 5)
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Overshot pH of Competent cell buffer (RbCl) :( - (reply: 3)
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digitonin lysis buffer - (reply: 1)
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Cloudy Alkaline Phosphatase Buffer! - my AP buffer gets cloudy after autoclave (reply: 6)
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Sodium phosphate buffer preparation - pH adjustment (reply: 5)
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Lysis buffer for luciferase assay - (reply: 1)
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1N NaOH solution can be autoclaved? - (reply: 9)
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Na Acetate buffer recipe - Na Acetate buffer (reply: 2)
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Need your suggestion about hybridization solution - The chemical reagants that contained in hybridization solution (reply: 4)
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Lysis Buffer for Elisa - (reply: 1)
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lipase activity assay - p-nitrophenyl myristate - Turbidity of my substrate solution (reply: 1)
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Free trial kit for DNA, RNA, and protein extraction - AquaRNA - an aqueous DNA, RNA, protein extraction solution (reply: 4)
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Stripping Solution: Recycle Anyone? - (reply: 6)
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How to clean the RNA buffer Tank (to get rid of RNase)? - (reply: 4)
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Can gel filtration effect buffer composition? - (reply: 4)
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cell lysis buffer - (reply: 4)
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Recombinant protein solution turns yellow when concentrated - (reply: 3)
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does anyone use pre-chilled SDS buffer? - (reply: 4)
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buffer preparation software - beta testers wanted - (reply: 5)
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How to choose the right buffer for my western blotting? - (reply: 2)
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PBS buffer for cell isolation sing dynabeads - (reply: 2)
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protein extraction - cytosolic lysis buffer (reply: 8)
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Why hepes buffer turned yellow - (reply: 4)
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Recipe for Impron II buffer (Promega) - We have excess enzyme and not enough buffer! (can't belive it myself) (reply: 2)
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Molarity of multicomponent buffer solutions - (reply: 3)
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Yellow Laemmli Sample Buffer - (reply: 15)
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selection of buffer for dialysis? - (reply: 1)
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Gummy mess after heating SDS-PAGE sample loading buffer - (reply: 2)
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How do I make a blocking buffer solution? - (reply: 1)
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Need help to sterilize a solution... - (reply: 6)
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Buffers for molecular work - Didn't know where to post (reply: 6)
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solution for dilute DNA - Dilution (reply: 1)
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TE buffer exposed in the air overnight...is it all right for use? - TO DISSOLVE DNA (reply: 7)
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Expiration date for Potassium phosphate buffer? - (reply: 1)
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Lysis buffer used to get cell lysates need to be sterilised? - (reply: 8)
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Reconstituting lyophilized oligonucelotides - How to make a stock solution with specific molarity, given with a cert (reply: 1)
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What is the function of acetate solution in DNA extraction for PCR used - (reply: 6)
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RIPA Buffer and DNA - (reply: 1)
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HEPES Kinase Assay Buffer - (reply: 2)
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Coating buffer for ELISA - Buffer composition needed...Help (reply: 2)
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WB reverse image solution?! - white band on darck background (reply: 2)
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FACS 5%FBS vs 1%BSA vs FACS Buffer - (reply: 5)
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Buffer for cation exchange chromatography - acetic acid or sodium acetate? - (reply: 1)
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Preparation of lysis buffer for enzyme extraction - (reply: 5)
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TRIS and PBS buffer with ERK (enterokinase) - ERK (enterokinase) (reply: 1)
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Cell lysis buffer for real time RT-PCR - Request for cell lysis buffer recipe or relevant scientific paper (reply: 1)
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How to quantify protein which has sds buffer already - (reply: 1)
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pmsf not dissolving in buffer - (reply: 3)
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How would I prepare a single phosphate buffered saline solution from three stock - (reply: 4)
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thrombin cleavage buffer - (reply: 1)
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Effects of a basic solution on photosynthesis - (reply: 3)
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do nanodrop blanking solutions affect readings? - (reply: 2)
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protein G, binding buffer pH? - (reply: 3)
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PBS buffer - (reply: 2)
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Is there ay staining solution to stain colony on agarose gel - (reply: 2)
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protein determination in loading buffer - (reply: 1)
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ChIP cell lysis buffer - what lysis buffer do you use for Chromatin IPs (reply: 6)
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Buffer Exchange & Protein Purification - (reply: 1)
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His-tag protein purification- buffers to use - What buffers to choose for His-tag chromatography (reply: 2)
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Na-K phosphate buffer recipe? - (reply: 2)
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storage buffer for purified protein - (reply: 3)
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RIPA as Extraction Buffer in ELISA - (reply: 3)
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how to convert molar solutions - (reply: 4)
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Making 0.2% acetic acid solution - (reply: 2)
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Suitable lysis buffer to extract transmembrane protein? (eg. tyrosinase) - (reply: 11)
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Stock solutions? - Does anyone use them?? (reply: 3)
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Enzymes in 10 x buffer - (reply: 2)
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what's the function of glycerol in co-ip buffer - (reply: 2)
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Why HEPES and folic acid in my lysis buffer? - (reply: 4)
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On trypsin neutralizing solution - (reply: 2)
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Do you add Triton-X in your lysis buffer? - (reply: 7)
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recover peptide in solution - question of how to recover peptide about 1500 Da from solution (reply: 1)
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pipetting sticky solutions - (reply: 15)
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Do you re-use SDS-PAGE running buffer? - (reply: 10)
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Protein crashing out of solution - (reply: 4)
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Homebrew Hybridization buffers - (reply: 2)
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Hybridization Buffer Won't DISSOLVE - (reply: 1)
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buffer for anion exchange chromatography of basic proteins - (reply: 1)
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how to define an appropriate protein reaction buffer? - (reply: 1)
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Sodium phosphate buffer - (reply: 5)
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About Tris-Cl buffer - (reply: 4)
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Two-color blocking buffer? BSA vs FBS - (reply: 1)
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Cloudy mowiol solution - (reply: 1)
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Solutions for manual RNA purification - (reply: 2)
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Biotin EMSA Blocking buffer - Search for a suitable blocking buffer (reply: 1)
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Troubleshoot SB buffer electrophoresis problem - (reply: 6)
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commercially available neutralized N-acetyl-L-cysteine solution? - (reply: 1)
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buffer preparation - (reply: 5)
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how to adjust the PH value of aminocaproic acid solution - (reply: 1)
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composition of Qiagen RDD buffer - (reply: 1)
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Do antibody solutions settle? - (reply: 2)
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Does 20x TTE buffer REALLY expire? - polyacryamide gels; buffers (reply: 1)
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when concentrating my protein sample my solution turns a yellow colour ? why? - (reply: 2)
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How to prepare thimerosal solution - (reply: 1)
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Organelle separation - buffer, homogenization, centrifugation force? - (reply: 1)
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SDS-PAGE Loading Buffer with Ficoll? - (reply: 6)
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buffer exchange problem - (reply: 3)
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Alternatives to Bradford - Bradford disturbed by Lysis Buffer (reply: 3)
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Lysis Buffer Preparations - (reply: 3)
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2x SDS-Loading Lysis buffer: use 2x or 1x? - (reply: 4)
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purpose of sucrose in buffer - organelle separation - (reply: 2)
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washing buffer retained in Ni-NTA column after centrifugation - (reply: 5)
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Temperature coefficient : caps buffer - (reply: 1)
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Buffer preparation - Problem with EDTA (reply: 3)
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Solution Making, Tris, and adjusting pH - before/after? - (reply: 6)
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lysis buffer for coip with a membrane protein - (reply: 2)
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Tris Tricine Gel Running Buffer - (reply: 1)
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Does this buffer suitable for DNA extraction? - (reply: 4)
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persistant bacterial contamination, causes and solutions - (reply: 8)
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androgen solution for cell treatment - (reply: 7)
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How to make EGTA solution? - (reply: 2)
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Why the heck my PBS solution is claudy? - I just made PBS adjusted to pH 7.5 (reply: 3)
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please give me some suggestions on preparating Yeast cells with PI stainning fo - buffer ,PI solution (reply: 4)
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DNA pellet won't go into solution - (reply: 4)
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What is buffer AVE ? - (reply: 3)
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BUFFER CALCULATION - (reply: 1)
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recipe for SB buffer for electrophoresis - (reply: 4)
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buffer components - (reply: 2)
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affinity purification of antibodies - what is the best buffer to elute (reply: 2)
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Help in making up a buffer - (reply: 13)
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BCl-2 lysis buffer - BCL-2 lysis buffer recipe (reply: 1)
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blocking solution of indirect immunostaining - many days in blocking solution (reply: 3)
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buffer for CD - (reply: 1)
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how does lysis solution - (reply: 3)
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Formaldehyde Fix cells to prevent detergent lysis ? - Testing a protocol to make cells resistant to cell lysis buffer (reply: 2)
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I added 2.5 times as much LB to TSS (Transformation and Storage Buffer) - What would happen? (reply: 1)
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As, MES - Storage of the solutions: As, MES (reply: 1)
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Storage of B glycerophosphate NaF , Na3VO4, Na4P2O7 stock solution for lysis buf - How to store the protease kinase inhibitor soluble stock for lysis buf (reply: 3)
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lysis buffer preparation - buffer preparation (reply: 6)
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Disinfection with weak solutions of methanol and ethanol? - (reply: 1)
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What dialysis buffer should I use for the protein subsequently to be used for ki - What decides the composition of the dialysis buffer? (reply: 1)
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calculation problem in preparing the phosphate buffer - (reply: 1)
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Low Salt Buffer - (reply: 5)
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preparation of Tris HCl buffer - (reply: 2)
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Buffer Exchange - (reply: 3)
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westerns, protein concentr and RIPA buffer - (reply: 2)
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SDS-PAGE sample buffer; SDS solubility - (reply: 2)
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Sodium Borate Buffer - (reply: 4)
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Buffers for MONO S purification - (reply: 1)
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transfer buffer question - anyone ever use TBS as a transfer buffer? (reply: 4)
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Standard Transfer Buffer Recipe - Western Blot Transfer Buffer (reply: 2)
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lactate buffer - needed composition of lactate buffer (reply: 2)
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NADH solution decays on its own? - (reply: 1)
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NADH buffer solution itself decays - (reply: 1)
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phenol chloroform isoamylalcohol - When do I add the TRIS buffer? (reply: 3)
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ELISA/ BSA in capture antibody storage buffer - (reply: 1)
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TCA precipitation, what is the best solution to dissolve followed by Mass spectr - (reply: 1)
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T4 DNA ligase Buffer - (reply: 3)
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Preparing Gel Loading Buffer - (reply: 6)
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Solution for a Genome Problem - Calculation of Genome Transformation Probability (reply: 1)
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Matrigel invasion assays. Need a fixing solution - (reply: 3)
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extraction buffer - (reply: 1)
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NP40 lysis buffer - (reply: 3)
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primer dissolved in wrong buffer (1M Tris HCl) - what to do now?? (reply: 7)
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LOADING BUFFER and UREA ? - (reply: 6)
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Removal of detergent from protein solution - (reply: 3)
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church buffer - (reply: 1)
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APS Solution Stability - Storage at -20°C vs. fresh solution preparations (reply: 11)
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problems with phosphate buffer in hydroxyl apatite chromatography - (reply: 1)
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denhardt's solution - (reply: 3)
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Primer Dilution. ...water or buffer? - (reply: 15)
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how to prepare Abeta for cell culture - how to the solution sterile (reply: 1)
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Making 0.5mM stock solution of BME - (reply: 3)
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pH of western blotting buffers - pH of western blotting buffers (reply: 3)
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Buffer QBT - (reply: 2)
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Western Blot Incubations - what solution is best for overnight incubations? (reply: 3)
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Protein lysis buffer for mass spec - recipe (reply: 15)
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Ultrafiltration of Enzyme Solution? - (reply: 4)
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Storage stability of hormone solutions - How would you prepare your working solution if your boss thinks that n (reply: 3)
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recipe of 0.1M Glycine-NaOH buffer - (reply: 2)
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0,050 M PHOSPHATE BUFFER - (reply: 9)
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BORATE BUFFER - (reply: 1)
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salt conc in lysis/homogenization buffer? - effect of different salt (NaCl) conc on cell lysis? (reply: 3)
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electrophoresis buffer become very hot - (reply: 11)
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Making miniprep solutions for spin columns - Now that we can reuse the columns... (reply: 21)
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Solution Preparation - (reply: 5)
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Buffer Calculator - Good little buffer calculator (reply: 11)
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Stringent wash buffer for Immunoprecipitation - What decides stringency of wash buffer when perform IP? (reply: 2)
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LDS vs. Laemmli sample buffer - (reply: 1)
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SDS Precipitation with 1% Lysis Buffer after 10' on ice - (reply: 1)
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Best lysis buffer for mammalian cells to do affinity chromatography? - (reply: 3)
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TEMS Buffer preparation - (reply: 1)
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What buffer should I incubate HRP secondary? - (reply: 3)
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How do you mix solutions in cuvettes - (reply: 5)
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Contamination of antibiotic solutions - (reply: 5)
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Running Buffer composition in SDS Page - (reply: 7)
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Digestion in sub-optimal buffer - (reply: 5)
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binding buffer in GST-pulldown - (reply: 1)
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cell dead after changing the medium to PBS buffer - (reply: 3)
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Electrophoresis buffer prep questions - (reply: 9)
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column chromatography - how to make the buffer input rate constant? (reply: 4)
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TE Buffer (what did I do wrong?) - (reply: 5)
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Solutions, solutions, solutions! - Conversion from %w/w to molar solution (reply: 10)
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krebs-ringer buffer preparation - (reply: 1)
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did NEBiolab change BamH1's buffer to buffer 3? - (reply: 2)
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ELISA - new kit without coating buffer - (reply: 3)
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why my freshly prepared 30% Acrylamide solution has precipitate? - (reply: 10)
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Difference bwtween BME and DTT in sample buffer for WB after IP - (reply: 10)
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RIPA buffer - orthovanadate - (reply: 3)
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PCR buffer and PCR efficiency - buffer pH and salt (reply: 2)
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dilution buffer for ap labeled 2nd antibody - (reply: 1)
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Milky solution after adding 100% ethanol - RNA extraction (reply: 6)
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question about plasmid transfection - (water solution or buffer solution?) (reply: 3)
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Mix for sequencing - Big Dye buffer necessary or not? (reply: 3)
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stable buffer at high pH - (reply: 6)
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Lysis buffer ... composition - what's the role of glycerol and/or sucrose? (reply: 2)
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BigDye® v1.1 & v3.1 5X Sequencing Buffer Makeup? - (reply: 1)
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preciptiate in Fermentas T4 ligase buffer - (reply: 1)
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Loading buffer - (reply: 3)
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why the buffer contains the EDTA and MnCL2 at the same time? - Help!!! (reply: 6)
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Protein pellet won't resuspend in buffer following acetone precipitation - (reply: 4)
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SDS-PAGE hi pH/hi Tris loading buffer runs funny - Do proteins generally run funny on Lammelli SDS-PAGE with high pH/high (reply: 1)
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Buffer BPTE for DNA electrophoresis - (reply: 3)
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regarding the 1X TE buffer - (reply: 5)
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Problem with Sodium Borate Buffer - (reply: 8)
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DNA isolation from RLT buffer - (reply: 1)
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Question about Tris-Hcl and PBS buffer - (reply: 5)
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use of Pierce stripping buffer in WB - (reply: 3)
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What should be the pH of the leamlli sample buffer? - (reply: 7)
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Sucrose in Lysis Buffer? - (reply: 11)
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Benedict's Solution question - I need answers to the questions about what the results might be (reply: 1)
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glycine or tris buffer - (reply: 5)
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Freezing cells in blocking buffer? Urgent! - (reply: 7)
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making Ficoll gel loading buffer - (reply: 1)
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Storage of buffers - (reply: 3)
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digesting DNA with buffer incompatible enzymes - (reply: 4)
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Why NaCl is in so many buffers? - (reply: 3)
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Hepes buffers turned brown after autoclave - Can I use it? (reply: 5)
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why does ligase buffer precipitate? - is it ok just to vortex it?? (reply: 12)
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Co-IP..... - What's important for the lysis buffer/washing buffer? (reply: 2)
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phosphate buffer.. - na/k/na-k? differences? choice? better? (reply: 2)
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buffer and molarity.. choice? - pH 7.0 (reply: 3)
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sp-cation exchange column.. - phosphate /tris buffer ph 7.0 (reply: 2)
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PH Calibration - Usage of Std Buffers (reply: 1)
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sterilization of buffer - (reply: 4)
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How to make 1X phosphate buffer, PB? - (reply: 4)
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silver stain can even get band with the loading buffer control - (reply: 4)
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Western blotting with room temperature buffers - protocol says NOT to use cold buffers (reply: 6)
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Trouble with Western blot transfer buffer - (reply: 11)
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Dose anyone know IP buffer recipe? - (reply: 6)
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Any protocol or methods for estimation of amines in solution - (reply: 1)
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effect of TE buffer on cells with cell lysis solution - (reply: 2)
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DTT in lämli buffer - (reply: 3)
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problem: buffer preparation-molecular biology - (reply: 5)
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Flag-tag purifaction elute w/ peptide or SDS loading buffer - (reply: 1)
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wash buffer vs lysis buffer PH 7.4 or 8.0 - (reply: 1)
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Fe SO4 stock solution - (reply: 7)
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Autoclave 1% Triton Solution? - (reply: 6)
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Effect of autoclave on solution concentration? - Buffer, sugar, salt (reply: 13)
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Purpose of High ionic strength buffers - (reply: 3)
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use of Tris-HCL in rehydration buffer - (reply: 1)
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Is my RNA dead? - freezing cells after adding RNA lysis buffer (reply: 2)
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Problem: High background using RIPA buffer with the bradford assay - (reply: 7)
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function of KRB buffer? - (reply: 4)
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Transfer Buffer - (reply: 7)
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Phosphate Buffer to adjust pH in LB - (reply: 3)
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Adenovirus Purification: CsCl solution sould be fresh? - Double CsCl Gradient (reply: 1)
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How long can RIPA buffer can be stored at 4C - (reply: 7)
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changing dialysis buffer - (reply: 7)
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Histidine in buffer, what is the role? - cytosolic and membrane protein isolation (reply: 1)
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Does anyone add Etbr to running buffer used to make agarose gels? - (reply: 14)
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1% SDS lysis buffer - (reply: 3)
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Filter sterilisation of phytohaemagglutinin solution - (reply: 1)
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Resuspended DNA in false buffer! - How to precipitate? (reply: 5)
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what these components do in the buffer used in protein purification - (reply: 5)
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protein folding- looking for renaturation buffer - (reply: 1)
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protein folding- looking for renaturation buffer - (reply: 1)
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role of ammonium sulphate in PCR buffer - (reply: 2)
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Qiagen gel extraction kit - strange buffer color (reply: 7)
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Hygromycin B Stock Solution - (reply: 3)
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reconstituted plasmid DNA solution slightly milky - (reply: 3)
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Buffer Baffled! - (reply: 3)
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An alternative to a PIPES buffer ... - how to dissolve and enzymatically determine lipids (reply: 3)
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DNA loading buffer - (reply: 11)
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pH buffer - make a pH buffer (reply: 2)
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Any database for roles of components in buffers? - (reply: 2)
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Buffer to stop grass yellowing from dog urine? - (reply: 6)
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Does SDS loading buffer + heat completely denature protein? - (reply: 3)
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Maybe dumb question - MgCl2 lysis buffer - (reply: 6)
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Electrode Storage solution - Protocol for preparation of Storage solution (reply: 3)
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Precipitate in PCR buffer w/BSA & 2-mercaptoethanol - PCR buffers (reply: 1)
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Why does changinf buffer effect isolation of RNA? - (reply: 1)
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Buffers for Anion Exchange Chromatogrphy - (reply: 8)
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Why is EB added into RNA loading buffer? - (reply: 4)
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erthrocyte lysis buffer - (reply: 2)
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Sorensen's Buffer - (reply: 3)
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cell lysis buffer recipe - simple question (reply: 6)
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Oxidation of HEPES Buffer Solutions - (reply: 1)
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Preparing blocking solution for Western Blotting - what milk power to use? - western blot (reply: 9)
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how to remove the foam of BSA solution - (reply: 2)
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cell lysis buffer for membrane protein please? - desperately need help.... :S (reply: 8)
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what is the function of the sodium azide in the buffer? - western blot (reply: 3)
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ABout the solution in Western blot and SDS? - (reply: 5)
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DTT in mammilian lysis buffer? - (reply: 12)
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temperature stable buffers? - Does snyone know a buffer which has a stable pH at various temperatures? (reply: 3)
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pH adjustment for potassium acetate solution - (reply: 1)
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E. coli Broth: Sodium phosphate verses Potassium phosphate buffer - (reply: 2)
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Making a solution - (reply: 6)
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Role of sodium azide in block solution of Western blot - (reply: 4)
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how to make this solution? - (reply: 13)
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Problem in making SDS-page Sample buffer - (reply: 7)
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Acidic buffer for short-time in vitro experiment - (reply: 2)
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Protein lysis buffer - Choice of buffer for different proteins (reply: 1)
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MSP BUFFER BY Herman et al., - (reply: 2)
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Question about stability of bacteria solution - (reply: 2)
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Unknown solution - (reply: 3)
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Biotinilated proteins. Problems with Hens buffer - (reply: 2)
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some protocol - buffer solution (reply: 2)
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Washing buffer - cold or warm? - (reply: 6)
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Why to use TE buffer for DNA resuspension - (reply: 2)
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3T3 cells and lysis buffer recepie - western blot (reply: 1)
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Role of Tris and Glycine in transfer buffer - what are the roles of tris and glycine in transfer buffer? (reply: 4)
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Glycerol for enzyme buffer - (reply: 4)
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lysozyme solution storage - (reply: 2)
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One or two bands with different protein extraction buffer - (reply: 3)
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What is the purpose of SDS in microarray hybridization buffers - (reply: 2)
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Phosphatase Inhibitors into IP lysis buffer! - (reply: 2)
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Concentrate protein from dilute solution - (reply: 2)
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what buffer will prevent the precipitation of protein at -20? - (reply: 5)
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What is the use of Koch's Lysis Solution? - Question (reply: 8)
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how to remove media components from protein solutions - (reply: 4)
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Diluent buffer for CIP phosphatase, glycerol conc? - (reply: 1)
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Northern Blots - can you reuse the buffers and membrane - Even if its dig labelled (reply: 5)
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Tris Buffers vs. pH Electrodes - (reply: 1)
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question about lysis buffer - (reply: 3)
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SEC for basic protein - column, buffer (reply: 4)
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WAHT IS TE BUFFER FOR? - (reply: 9)
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Will antibody need any buffer to make link with bead - Which buffer should I use to link bead with antibody (reply: 1)
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Silver Staining Protocol for DNA - EDTA solution concentration missing (reply: 1)
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buffer for His-tag recombinant protein purification - (reply: 2)
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can't stop the precipitate! - adding cacl2 to pk buffer (reply: 6)
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RIPA buffer mistake - (reply: 2)
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Added DNTP solution to DNA by mistake - (reply: 3)
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bacterial pellet and lysis buffer - (reply: 4)
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Crystallisation of Phenol saturated solution - (reply: 2)
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How to swap buffers? - going from tris-hcl to potassium phosphate (reply: 1)
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Question about solution preparation - (reply: 4)
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Making buffers - (reply: 5)
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concentrating protein from different lysis buffers? - Can you concentrate proteins from differnt buffers and resuspend all in the same (reply: 5)
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lysis buffer to extract transmembrane protein - (reply: 3)
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Protein quantification: BCA with laemmli buffer? - does it work? (reply: 11)
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Difference between salt solution? - (reply: 1)
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Problem with DNA extraction from plants - CTAB solution and nanodrop results (reply: 3)
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DNA extraction problem - CTAB solution (reply: 2)
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interactin between alkaline phosphatase buffer and PNK buffer. - help please (reply: 1)
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Whole cell extraction buffer... - does any one have the recipe? (reply: 1)
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ELISA wash buffer alternates - (reply: 2)
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How to control pH in electrophoresis-----the problem of the buffer - (reply: 2)
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SDS sample buffer and serum - why is it solidifying? (reply: 3)
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Double digest: efficiency - what does 50% active in given buffer mean? (reply: 1)
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Please recommend some solutions for size-exclusion chromatography - (reply: 4)
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solution problem - (reply: 5)
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Restriction buffer - (reply: 3)
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Acrylamide stock for gels - which buffer (reply: 6)
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Osmoprotectant for Strep. mutans and buffer - (reply: 1)
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SDS and transfer buffer......... - (reply: 1)
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about G418 and neomycin - is there any difference? the solution? (reply: 10)
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buffer for expression analysis - (reply: 3)
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buffer composition - (reply: 11)
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Phosphate Buffer - how much buffer to add wastewater (reply: 6)
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hepes solution storage - (reply: 4)
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PVP: Polyvinylpyrollidone - protein extraction buffer (reply: 3)
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strange western - transfer turned PVDF, filter, sponge, and buffer yellow? (reply: 6)
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Preparing potassium phosphate buffer(pH 7.4) - (reply: 11)
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(Alcian Blue) Staining Solution Preparation - (reply: 1)
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mops buffer - (reply: 2)
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assay buffers - (reply: 1)
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buffer pH unsteady - (reply: 4)
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Trouble growing bacteria in LB/amp solution - (reply: 5)
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PCR buffers for Taq and Pfu polymerase - (reply: 6)
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Making a 80% ethanol/ 20% glacial acetic acid solution - (reply: 3)
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Can't make a borate solution. Ideas? - (reply: 4)
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problems in concentration protein solution - (reply: 8)
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Help me! I need the acidic buffer - (reply: 2)
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Guanidine-HCl solution storage - (reply: 1)
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loose pellet in prep of electr-competent E.coli - inquire for a solution to solve the problem of loose pellet (reply: 4)
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DEPC before or after solution preparation?... - (reply: 4)
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function of LiCl in ChIP wash buffer - (reply: 1)
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Does this qualify as potassium phosphate buffer? - (reply: 3)
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How much dialysis solution? - (reply: 1)
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for protein interaction what lysis buffer I should use? - (reply: 1)
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can I freeze my samples in loading buffer and use them the next day? - (reply: 2)
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How to choose HindIII/EcoRI double digest buffer? - a stupid question, but I really curious about the answer (reply: 6)
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TRS Buffer - whats in it?!? - Whats in Dako's TRS? (reply: 1)
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Agarose gels and SB Buffer - (reply: 7)
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SSC+NaPO4 buffers - (reply: 2)
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How to make aurintricarboxylic acid (ATA) solution? - (reply: 1)
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Borate buffer recipe - (reply: 14)
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question about phosphate buffer? - (reply: 1)
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NAD(P)H in solution - handling? - NAD(P)H be frozen and thawed for use in enzymatic assay? (reply: 2)
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Co-IP, nuclear proteins - buffers for lysis? - Who else does co-ip on nuclear proteins? (reply: 1)
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acetate buffer - (reply: 2)
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Phosphate Buffer - (reply: 5)
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MSP buffer composition - (reply: 1)
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Hank's balanced salt solution, do I need it? - Will PBS do instead? (reply: 3)
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preparation of bicine buffer? - (reply: 1)
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Water or TE buffer for dissolving DNA - (reply: 5)
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Plant DNA celan up - Recipie for clean up solution (reply: 2)
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good buffers - (reply: 1)
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Difference between adding NaOH to TBE buffer? - (reply: 4)
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concentration of SDS loading buffer ... - 1X buffer seems too little. (reply: 8)
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calculation for micromolar solution - (reply: 2)
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Inoue Methode - How to make the Buffer for the Inou eMethode (reply: 3)
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Role of common compounds in different buffers and solutions - compounds are acting in solution as... (reply: 17)
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TAE BUFFER - (reply: 2)
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science behind commonly used solutions - (reply: 1)
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Ambion low conductivity electroporation buffer? - (reply: 1)
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Dissolving DNA in TE buffer - is heating necessary? - (reply: 4)
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buffer problems - (reply: 1)
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how to prepare 0.066 M phosphate buffer? - preparation of buffer (reply: 3)
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Potassium phosphate buffer - (reply: 2)
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Lysis buffer - (reply: 3)
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Buffer for amonium sulphate precipitation - (reply: 1)
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how to remove salt from a sugar solution - (reply: 3)
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Filtering solutions - (reply: 7)
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decontamination of electrophoresis chambers... - what solution should be used? (reply: 2)
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Lysis buffer with 1% tritonX - Kindly sugget me its other ingredients (reply: 1)
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reducing and nonreducing buffer, SDS-PAGE - (reply: 3)
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Gel loading buffer - (reply: 12)
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How to accurately quantify plasmid DNA in solution within impurity of RNA? - (reply: 3)
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help with buffer 5.1 for RNA extraction - (reply: 6)
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Preparing McIlvaine buffer added with potassium chloride - buffer system (reply: 1)
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How to concentrate protein that already in SDS buffer and measure concentration? - Help please (reply: 4)
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Buffer preparation - Preparation of sodium phospate buffers (reply: 2)
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Why do we use methanol in the transfer buffer? - Hope somebody can send me a reply in why do we use methanol in the transfer buff (reply: 3)
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Need Help ASAP with maxiprep - Diluted plasmid with buffer ER instead of TE (qiagen kit) (reply: 3)
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Role of buffer in antibody purification - (reply: 3)
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buffer preparation - (reply: 3)
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Strange nanodrop readings after RNA isolation, strange solution - (reply: 13)
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water instead of TE buffer to dissolved primers - (reply: 6)
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Passive lysis buffer for luciferase assays - (reply: 11)
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Making TE buffer - (reply: 6)
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to prepare westen blot transfer buffer, instead of methanol can i use ethanol. - (reply: 3)
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Condition for sonication of protein solution to get rid of DNA? - How long to sonicate and with what amplitude? (reply: 4)
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How to remove LPS from protein solution - (reply: 2)
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how to store fixing solution with (para)formaldehyde - (reply: 2)
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IP lysis buffer formulation : why adding a non-ionic detergent if a ? - (reply: 4)
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Formulation of a buffer solution - (reply: 2)
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How to make malonate buffer? - (reply: 1)
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How to remove salt from a sugar solution? - Removing KH2PO4 from glucose solution (reply: 7)
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DNA, RNA and protein extraction using basic solution - (reply: 4)
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Qiagen RPE buffer - (reply: 1)
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Why is TE frequently used as buffer for DNA solutions? - (reply: 1)
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PBS buffer + HEPES buffer - can be mixed together? - Can I mix two protein in different buffers, same pH? (reply: 2)
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PstI restrictase (Invitrogen) - reaction buffer (reply: 1)
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Making Tris Buffers of Various pH's by Mixing Tris acid and Tris Base - No HCL needed (reply: 5)
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Annealing buffer for DNA oligos - RNA interference (reply: 2)
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Collagen solution for coating: do you freeze it? - 4 mg/ml Biochrom AG ready solution (reply: 1)
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Ampicillin - Problem in amipicillin solution prep. (reply: 13)
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Developing solution at northern blot - (reply: 1)
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STRIPPING BUFFER? - how to make good STRIPPING BUFFER? (reply: 3)
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why use 2X versus 4X sample buffer? - (reply: 6)
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50mM HEPES-KOH buffer pH7.4 formulation please - (reply: 1)
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lysis buffer - (reply: 3)
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Lysis Buffer Preparation - (reply: 3)
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Can't get PVP into solution - (reply: 1)
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FUNCTION OF BUFFER - (reply: 3)
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'Blue loading buffer' - (reply: 6)
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Emergent: Toxicity of cell lysis buffer :( - (reply: 2)
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Can I add additional sodium ion to PCR reaction buffers? - (reply: 5)
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8-MOP solution - (reply: 2)
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how many times do you re-use these two buffers? - (reply: 5)
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Role of restriction and TAE Buffer - (reply: 1)
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antibody in SDS-containing solution - (reply: 3)
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HEPES-KOH buffer - (reply: 5)
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digestion with two REs requiring different buffer - (reply: 10)
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NMDA buffer toxic? - (reply: 8)
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Why freshly prepared alkaline solution II for plasmid extraction? - (reply: 10)
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Change Tris for Phosphate buffer cause precipitation - Which buffer can replace Tris? (reply: 3)
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Water as an alternative for TE buffer? - (reply: 11)
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Stop Solution Doesn't Stop the Reaction - (reply: 3)
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His Tag purif buffer-to Tris or not to Tris? - best buffer for pH=7.0 (reply: 1)
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buffer preparation for molecular biology - (reply: 9)
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Difference between phosphate buffer & potassium phosphate buffer - (reply: 2)
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what is the function of Na2EDTA in a buffer solution - (reply: 2)
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50mM Potassium Phosphate buffer, pH 6.0 - (reply: 1)
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0.1M Phospahte Buffer, pH8.5 - Buffer Protocol (reply: 1)
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pH balance - HCO3/CO2 buffer in culture media (reply: 1)
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loading buffer concentration - (reply: 2)
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Proteinase K activation/preparation - water or buffer in proteinase K (reply: 3)
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buffer exchange - (reply: 1)
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Phosphate buffer - (reply: 6)
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Storing of Tris/HCl buffer - (reply: 8)
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sterilizing protein solutions + agar - how to sterilize sensitive media (reply: 4)
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How to prepare potassium phosphate buffer - (reply: 6)
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How To Make 2M Bisulfite Solution? - Bisulfite Sequencing (reply: 6)
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orthovanadate in transfer buffer for WB? - immunodetection of phospho ERK1/2 (reply: 6)
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TAE Buffer - (reply: 4)
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10% CHAPS solution (10ml) - Recipe wrong or right? (reply: 1)
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Stripping Buffer Solution - Stripping Buffer Solution (reply: 3)
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phosphate buffer confusion - the true way of preparation pls??!! (reply: 1)
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Reuse TAE buffer? - (reply: 6)
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Making solutions for protein prep - (reply: 3)
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How to prepare 4M ammonium sulphate solution? - Ammonium sulphate precipitation (reply: 5)
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2-mercaptoethanol and TEMED solutions - (reply: 1)
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Reducing agent or not in SDS_PAGE sample buffer? - (reply: 2)
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shelf life of PMSF/ leupeptin in buffer - (reply: 1)
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Reference to Cheeney buffer - (reply: 1)
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DNAse and NaF in lysis buffer...a good idea? - Does NaF or Na3VO4 inactivate DNAse I? (reply: 2)
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problem with stripping membranes of western blott - do you know some efficient stripping buffer (reply: 3)
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What is the role for gelatin in PCR buffer - (reply: 1)
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DNase I treatment of RNA dissolved in TE buffer - (reply: 2)
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can i autoclave agarose gel with TAE buffer - (reply: 4)
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Buffer conditions for Pfu? - (reply: 3)
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SDC in buffer? - (reply: 2)
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Lysis buffers for brain tissue samples - Which ingredients are superfluous? (reply: 3)
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PPT in T4 DNA ligase buffer - (reply: 5)
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How to make phosphate buffer? - (reply: 10)
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Lysis buffer for membrane protien - (reply: 1)
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pH and types of containers for solution storage - (reply: 3)
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What is the pH of 2x loading buffer? - (reply: 1)
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Buffers for storing and diluting of dNTP - (reply: 3)
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TIssue Homogenization Buffer W/o DTT - Missing Reagent (reply: 1)
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RIPA lysis buffer (urgent Please) - Na-deoxycholate substitution (reply: 1)
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How to prepare primer solution - (reply: 7)
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How to increase the strigency of the washing buffer for IP? - (reply: 4)
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Problem w/ ECL homemade solution for WB - Final solution works only for a few minutes! (reply: 7)
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How to make Britton Robinson buffer? - Britton Robinson buffer receipt (reply: 3)
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Which buffers for storing of RNA and DNA? - (reply: 6)
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Western Blot: Reducing Buffer Vs Non-erducing buffer - basic Western blot question. (reply: 4)
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Is it ok to re-use Western Transfer Buffer - (reply: 4)
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protein suspended in diff buffer changes mobility - Will differnt salt concentration in buffer interfe (reply: 2)
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Gel extraction and elution buffer - (reply: 4)
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separation of nucleus protein - lysis buffer (reply: 1)
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Recipe for anti-fade solution?? - (reply: 1)
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can i autoclave buffers with imidazole for His-tag purification? - (reply: 2)
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His-taged protein is in 8M Urea, how to get rid of urea? - Ni-NTA column purified protein in 8M urea buffer (reply: 3)
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In situ Hybridization problem with TCS solution?? - (reply: 1)
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Help: western blot lysis buffer - (reply: 2)
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Lysis buffers for extraction of proteins expressed in E.coli - (reply: 5)
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SDS-PAGE loading buffer - 1X, 2X, 4X loading buffer? (reply: 2)
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Dissolving DNA in TE buffer - troubleshooting - (reply: 3)
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lysis buffer for western blot - lysis buffer (reply: 3)
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a recipe of a lysis buffer for co-IP (phosphorylation) - can anyone recommend one? (reply: 3)
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what these chemicals for in lysis buffer? - (reply: 3)
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Is TE buffer toxic to cultured cells? - cell culture (reply: 4)
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About the maleic acid buffer - Maleic acid buffer can be autoclave? (reply: 1)
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extraction buffer - (reply: 1)
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Phosphate buffers - protein and NaCl affecting pH of buffer (reply: 4)
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which buffer need to autoclave, which can not - (reply: 1)
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preparation of solutions that need pH adjustment - (reply: 3)
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NP40 concentration in protein lysis buffer - (reply: 1)
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Balanced salt solutions - (reply: 2)
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smear of loading buffer observed - SDS-PAGE gel (reply: 5)
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How do you prepare PCR stock solution to minimize pipetting - (reply: 37)
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How to make a tetracycline solution - (reply: 2)
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Plasmids in eluting buffer - (reply: 1)
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Make a solution of 10,000ppm hypochlorite - (reply: 9)
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preparing buffer - (reply: 3)
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MMP assay - proteases in lysis buffer (reply: 1)
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Azacytidine mother solution - (reply: 5)
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dsOligos annealing buffer - (reply: 2)
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Buffer for red blood cell lysis for RNA extraction from blood - RNA methodology (reply: 6)
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Sample Buffer Recipe (Laemmili) - (reply: 5)
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Good buffer needed! - (reply: 7)
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Can I use 4M urea buffer in DEAE chromatography? - (reply: 1)
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How to prepare solution for drug or hormone treatment?? - Preparing dexamethasone and estradiol solution from powder (reply: 4)
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Making TE buffer for RNA disolution - afraid of RNase contamination (reply: 3)
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Difference between TE buffer at pH 8.0 and 7.5 - (reply: 2)
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Protein precipitated after buffer exchange - (reply: 3)
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Reuse TAE buffer and agarose gel? - yes or no... (reply: 22)
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Coating Buffer for Sandwich ELISA - please help! (reply: 7)
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Alginic acid - How to get it into solution? (reply: 4)
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About 5' aza-cytidine - stock solution (reply: 2)
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Do enzymes work in different buffers? - (reply: 2)
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MES Buffer Receipe - (reply: 2)
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hydroproline assay citrate buffer - (reply: 1)
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SDS electrod buffer - SDS electrod buffer (reply: 2)
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Convert DNase units to weight - 2mg/ml from a 2U/ml solution (reply: 3)
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Electroblotting of Proteins on - transfer buffer without Tris and Glycine (reply: 1)
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Preparation of Estrogen solution - (reply: 2)
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Na2Hpo4 , NaH2po4 or both in Church buffer ?! - (reply: 2)
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can RIPA buffer use to prepare nuclear protein? - (reply: 1)
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Concentrating proteins from solution - (reply: 2)
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How to prepare the balance solution - (reply: 1)
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Ammonium Sulfate PCR buffer - Ammonium Sulfate PCR buffer (reply: 1)
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Lysis Buffer for MTT - (reply: 1)
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Fura 2 loading - Fura 2 /AM solution in DMSO loading in Tcells (reply: 1)
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what solution to use for cell lysis - (reply: 5)
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Chromatofocuusing - How to make a pH gradiem (3-8) by using simple buffers. (reply: 1)
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endotoxin free buffers - (reply: 1)
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Hank's solution - (reply: 2)
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Buccal swab storage - is saline solution enough? (reply: 1)
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electrophoresis buffer - when I should replace running buffer (reply: 1)
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cell lysis - How do I decide what solution to use? (reply: 2)
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What is the difference between 2x and 4x sample buffer? - SDS Sample buffer (reply: 1)
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MES buffer in dialyzation - MES or any alternative for MES? (reply: 1)
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solution lysis - (reply: 2)
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Why use imidazole as a buffer? - Imidazole Buffer (reply: 2)
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How to make PCR using the gel solution - (reply: 1)