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Buffer Related Discussions
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Dilution Buffer for Elisa - Dilution buffer for titre check of polyclonal serum or for monoclonal (reply: 2)
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NaPi buffer - NaPi buffer recipie (reply: 1)
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Ligation buffer - DTT in ligation buffer (reply: 1)
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Glycine in blocking solution - (reply: 3)
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1st strand buffer for cDNA synthesis - (reply: 1)
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Concentration of EDTA in lysis buffer - (reply: 1)
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how to prepare phosphate buffer - 1M phospahate buffer (reply: 1)
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Tris Hcl running gel buffer problems - (reply: 6)
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RNA samples precipitates when I add 10X Buffer for Turbo Dnase treatment - RNA precipitation (reply: 2)
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How to prepare buffer solutions? - I need some help here! (reply: 3)
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separation of loading buffer - (reply: 1)
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recipe for LDS based sample buffer - (reply: 1)
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Volatile Buffers for pH 3 - 10 - Generating a list of volatile buffers for mass spectrometry (reply: 1)
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EDTA and EGTA into solution - EDTA and EGTA into solution (reply: 5)
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cell lysis buffer to use directly on the dish - (reply: 1)
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Proteins recover from different buffer - How to obtain again proteins from ST buffer? (reply: 2)
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SDS buffers - (reply: 1)
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SDS buffer - (reply: 1)
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When to use PMSF in a cell lysis buffer? - (reply: 9)
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Why 150 mM KCL in homogenization buffer - (reply: 7)
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8M urea buffer for protein extraction - (reply: 6)
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Buffer storage question - (reply: 2)
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Dissolve siRNA in non-RNase free buffer - (reply: 1)
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Powdered acrylamide versus solution acrylamide - (reply: 13)
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SDS buffer - (reply: 2)
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Cell Lysis buffer....do I have to add sodium phosphate to the buffer? - lysis buffer for DNA extractions (reply: 4)
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Wash solution for Fura-2 AM - (reply: 2)
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pH lysis buffer - (reply: 1)
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Filtration of Acrylamide Solution - (reply: 1)
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taq Gold and rox buffer - (reply: 2)
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Cell count in solution - (reply: 2)
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Dilution Problem! - Making up molar solutions.... (reply: 8)
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IP buffer RIPA - Preserve buffer (reply: 3)
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Storage of solutions - (reply: 5)
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Stripping buffer - (reply: 5)
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Multigene cloning - Looking for a cheap solution - (reply: 4)
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transfer buffer - (reply: 1)
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How to sterilize Ovalbumin solution? - (reply: 3)
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Why is stock solution needed to be sterilized? - Calcium chloride, and Tween 80 ok after autoclave? (reply: 10)
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About Hank's balanced salt solution - (reply: 3)
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buffer preparation - (reply: 3)
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Washing buffer and sauton medium - Survival assay in poor condition (reply: 3)
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asking for kanamycin stock solution... - (reply: 4)
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protein band is shifted after boiling sample with sample buffer - (reply: 4)
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Sample Buffer - (reply: 1)
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Dialysis Buffer for Recombinant Protein - (reply: 5)
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FACS sorting into what solution for RNA isolation? - (reply: 2)
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PBS and STE Buffer - (reply: 2)
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phosphate buffer - (reply: 2)
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RNA gel loading buffer? - (reply: 5)
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Tris or Hepes? - buffer choice (reply: 1)
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how to prepare 0.1 M sodium acetate buffer, pH 5 - i'm bad with my chemistry! (reply: 2)
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DMSO in western lysis buffer - (reply: 1)
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treatment buffer concentration - (reply: 5)
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digitonin lysis buffer - (reply: 1)
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Sodium phosphate buffer preparation - pH adjustment (reply: 5)
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1N NaOH solution can be autoclaved? - (reply: 13)
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Need your suggestion about hybridization solution - The chemical reagants that contained in hybridization solution (reply: 4)
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lipase activity assay - p-nitrophenyl myristate - Turbidity of my substrate solution (reply: 1)
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Stripping Solution: Recycle Anyone? - (reply: 6)
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Can gel filtration effect buffer composition? - (reply: 4)
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Recombinant protein solution turns yellow when concentrated - (reply: 3)
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buffer preparation software - beta testers wanted - (reply: 5)
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PBS buffer for cell isolation sing dynabeads - (reply: 2)
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Why hepes buffer turned yellow - (reply: 4)
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Molarity of multicomponent buffer solutions - (reply: 3)
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selection of buffer for dialysis? - (reply: 1)
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How do I make a blocking buffer solution? - (reply: 1)
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Buffers for molecular work - Didn't know where to post (reply: 6)
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TE buffer exposed in the air overnight...is it all right for use? - TO DISSOLVE DNA (reply: 7)
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Lysis buffer used to get cell lysates need to be sterilised? - (reply: 8)
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What is the function of acetate solution in DNA extraction for PCR used - (reply: 6)
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HEPES Kinase Assay Buffer - (reply: 2)
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WB reverse image solution?! - white band on darck background (reply: 2)
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Buffer for cation exchange chromatography - acetic acid or sodium acetate? - (reply: 1)
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TRIS and PBS buffer with ERK (enterokinase) - ERK (enterokinase) (reply: 1)
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How to quantify protein which has sds buffer already - (reply: 2)
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How would I prepare a single phosphate buffered saline solution from three stock - (reply: 4)
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Effects of a basic solution on photosynthesis - (reply: 3)
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protein G, binding buffer pH? - (reply: 3)
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Is there ay staining solution to stain colony on agarose gel - (reply: 2)
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ChIP cell lysis buffer - what lysis buffer do you use for Chromatin IPs (reply: 6)
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His-tag protein purification- buffers to use - What buffers to choose for His-tag chromatography (reply: 2)
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storage buffer for purified protein - (reply: 3)
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how to convert molar solutions - (reply: 4)
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Suitable lysis buffer to extract transmembrane protein? (eg. tyrosinase) - (reply: 11)
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Enzymes in 10 x buffer - (reply: 2)
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Why HEPES and folic acid in my lysis buffer? - (reply: 4)
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Do you add Triton-X in your lysis buffer? - (reply: 7)
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pipetting sticky solutions - (reply: 15)
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Protein crashing out of solution - (reply: 4)
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Hybridization Buffer Won't DISSOLVE - (reply: 1)
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how to define an appropriate protein reaction buffer? - (reply: 1)
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About Tris-Cl buffer - (reply: 4)
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Cloudy mowiol solution - (reply: 1)
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Biotin EMSA Blocking buffer - Search for a suitable blocking buffer (reply: 1)
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commercially available neutralized N-acetyl-L-cysteine solution? - (reply: 1)
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how to adjust the PH value of aminocaproic acid solution - (reply: 1)
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Do antibody solutions settle? - (reply: 2)
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when concentrating my protein sample my solution turns a yellow colour ? why? - (reply: 2)
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Organelle separation - buffer, homogenization, centrifugation force? - (reply: 1)
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buffer exchange problem - (reply: 3)
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Lysis Buffer Preparations - (reply: 3)
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purpose of sucrose in buffer - organelle separation - (reply: 2)
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Temperature coefficient : caps buffer - (reply: 1)
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Solution Making, Tris, and adjusting pH - before/after? - (reply: 6)
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Tris Tricine Gel Running Buffer - (reply: 1)
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persistant bacterial contamination, causes and solutions - (reply: 8)
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How to make EGTA solution? - (reply: 2)
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please give me some suggestions on preparating Yeast cells with PI stainning fo - buffer ,PI solution (reply: 4)
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What is buffer AVE ? - (reply: 3)
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recipe for SB buffer for electrophoresis - (reply: 4)
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affinity purification of antibodies - what is the best buffer to elute (reply: 2)
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BCl-2 lysis buffer - BCL-2 lysis buffer recipe (reply: 1)
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buffer for CD - (reply: 1)
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Formaldehyde Fix cells to prevent detergent lysis ? - Testing a protocol to make cells resistant to cell lysis buffer (reply: 2)
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As, MES - Storage of the solutions: As, MES (reply: 1)
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lysis buffer preparation - buffer preparation (reply: 6)
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What dialysis buffer should I use for the protein subsequently to be used for ki - What decides the composition of the dialysis buffer? (reply: 1)
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Low Salt Buffer - (reply: 5)
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Buffer Exchange - (reply: 3)
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SDS-PAGE sample buffer; SDS solubility - (reply: 2)
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Buffers for MONO S purification - (reply: 1)
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Standard Transfer Buffer Recipe - Western Blot Transfer Buffer (reply: 2)
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NADH solution decays on its own? - (reply: 1)
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phenol chloroform isoamylalcohol - When do I add the TRIS buffer? (reply: 3)
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TCA precipitation, what is the best solution to dissolve followed by Mass spectr - (reply: 1)
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Preparing Gel Loading Buffer - (reply: 6)
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Matrigel invasion assays. Need a fixing solution - (reply: 3)
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NP40 lysis buffer - (reply: 3)
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LOADING BUFFER and UREA ? - (reply: 6)
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church buffer - (reply: 1)
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problems with phosphate buffer in hydroxyl apatite chromatography - (reply: 1)
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Primer Dilution. ...water or buffer? - (reply: 15)
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Making 0.5mM stock solution of BME - (reply: 3)
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Buffer QBT - (reply: 2)
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Protein lysis buffer for mass spec - recipe (reply: 15)
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Storage stability of hormone solutions - How would you prepare your working solution if your boss thinks that n (reply: 3)
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0,050 M PHOSPHATE BUFFER - (reply: 9)
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salt conc in lysis/homogenization buffer? - effect of different salt (NaCl) conc on cell lysis? (reply: 3)
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Making miniprep solutions for spin columns - Now that we can reuse the columns... (reply: 21)
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Buffer Calculator - Good little buffer calculator (reply: 11)
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LDS vs. Laemmli sample buffer - (reply: 1)
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Best lysis buffer for mammalian cells to do affinity chromatography? - (reply: 3)
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What buffer should I incubate HRP secondary? - (reply: 3)
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Contamination of antibiotic solutions - (reply: 5)
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Digestion in sub-optimal buffer - (reply: 5)
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cell dead after changing the medium to PBS buffer - (reply: 3)
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column chromatography - how to make the buffer input rate constant? (reply: 4)
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Solutions, solutions, solutions! - Conversion from %w/w to molar solution (reply: 10)
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did NEBiolab change BamH1's buffer to buffer 3? - (reply: 2)
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why my freshly prepared 30% Acrylamide solution has precipitate? - (reply: 10)
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RIPA buffer - orthovanadate - (reply: 3)
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dilution buffer for ap labeled 2nd antibody - (reply: 1)
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question about plasmid transfection - (water solution or buffer solution?) (reply: 3)
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stable buffer at high pH - (reply: 6)
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BigDye® v1.1 & v3.1 5X Sequencing Buffer Makeup? - (reply: 1)
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Loading buffer - (reply: 3)
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Protein pellet won't resuspend in buffer following acetone precipitation - (reply: 4)
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Buffer BPTE for DNA electrophoresis - (reply: 3)
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Problem with Sodium Borate Buffer - (reply: 8)
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Question about Tris-Hcl and PBS buffer - (reply: 5)
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What should be the pH of the leamlli sample buffer? - (reply: 7)
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Benedict's Solution question - I need answers to the questions about what the results might be (reply: 1)
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Freezing cells in blocking buffer? Urgent! - (reply: 7)
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Storage of buffers - (reply: 3)
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Why NaCl is in so many buffers? - (reply: 3)
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why does ligase buffer precipitate? - is it ok just to vortex it?? (reply: 12)
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phosphate buffer.. - na/k/na-k? differences? choice? better? (reply: 2)
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sp-cation exchange column.. - phosphate /tris buffer ph 7.0 (reply: 2)
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sterilization of buffer - (reply: 4)
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silver stain can even get band with the loading buffer control - (reply: 4)
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Trouble with Western blot transfer buffer - (reply: 11)
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Any protocol or methods for estimation of amines in solution - (reply: 1)
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DTT in lämli buffer - (reply: 3)
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Flag-tag purifaction elute w/ peptide or SDS loading buffer - (reply: 1)
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Fe SO4 stock solution - (reply: 7)
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Effect of autoclave on solution concentration? - Buffer, sugar, salt (reply: 13)
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use of Tris-HCL in rehydration buffer - (reply: 1)
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Problem: High background using RIPA buffer with the bradford assay - (reply: 7)
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Transfer Buffer - (reply: 7)
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Adenovirus Purification: CsCl solution sould be fresh? - Double CsCl Gradient (reply: 1)
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changing dialysis buffer - (reply: 7)
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Does anyone add Etbr to running buffer used to make agarose gels? - (reply: 14)
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Filter sterilisation of phytohaemagglutinin solution - (reply: 1)
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what these components do in the buffer used in protein purification - (reply: 5)
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protein folding- looking for renaturation buffer - (reply: 1)
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Qiagen gel extraction kit - strange buffer color (reply: 7)
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reconstituted plasmid DNA solution slightly milky - (reply: 3)
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An alternative to a PIPES buffer ... - how to dissolve and enzymatically determine lipids (reply: 3)
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pH buffer - make a pH buffer (reply: 2)
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Buffer to stop grass yellowing from dog urine? - (reply: 6)
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Maybe dumb question - MgCl2 lysis buffer - (reply: 6)
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Precipitate in PCR buffer w/BSA & 2-mercaptoethanol - PCR buffers (reply: 1)
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Buffers for Anion Exchange Chromatogrphy - (reply: 8)
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erthrocyte lysis buffer - (reply: 2)
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cell lysis buffer recipe - simple question (reply: 6)
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Preparing blocking solution for Western Blotting - what milk power to use? - western blot (reply: 11)
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cell lysis buffer for membrane protein please? - desperately need help.... :S (reply: 8)
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ABout the solution in Western blot and SDS? - (reply: 5)
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temperature stable buffers? - Does snyone know a buffer which has a stable pH at various temperatures? (reply: 3)
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E. coli Broth: Sodium phosphate verses Potassium phosphate buffer - (reply: 2)
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Role of sodium azide in block solution of Western blot - (reply: 4)
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Problem in making SDS-page Sample buffer - (reply: 7)
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Protein lysis buffer - Choice of buffer for different proteins (reply: 1)
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Question about stability of bacteria solution - (reply: 2)
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Biotinilated proteins. Problems with Hens buffer - (reply: 2)
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Washing buffer - cold or warm? - (reply: 6)
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3T3 cells and lysis buffer recepie - western blot (reply: 1)
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Glycerol for enzyme buffer - (reply: 4)
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One or two bands with different protein extraction buffer - (reply: 3)
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Phosphatase Inhibitors into IP lysis buffer! - (reply: 2)
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what buffer will prevent the precipitation of protein at -20? - (reply: 5)
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how to remove media components from protein solutions - (reply: 4)
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Northern Blots - can you reuse the buffers and membrane - Even if its dig labelled (reply: 5)
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question about lysis buffer - (reply: 3)
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WAHT IS TE BUFFER FOR? - (reply: 9)
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Silver Staining Protocol for DNA - EDTA solution concentration missing (reply: 1)
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can't stop the precipitate! - adding cacl2 to pk buffer (reply: 6)
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Added DNTP solution to DNA by mistake - (reply: 3)
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Crystallisation of Phenol saturated solution - (reply: 2)
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Question about solution preparation - (reply: 4)
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concentrating protein from different lysis buffers? - Can you concentrate proteins from differnt buffers and resuspend all in the same (reply: 5)
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Protein quantification: BCA with laemmli buffer? - does it work? (reply: 11)
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Problem with DNA extraction from plants - CTAB solution and nanodrop results (reply: 3)
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interactin between alkaline phosphatase buffer and PNK buffer. - help please (reply: 1)
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ELISA wash buffer alternates - (reply: 2)
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SDS sample buffer and serum - why is it solidifying? (reply: 3)
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Please recommend some solutions for size-exclusion chromatography - (reply: 4)
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Restriction buffer - (reply: 3)
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Osmoprotectant for Strep. mutans and buffer - (reply: 1)
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about G418 and neomycin - is there any difference? the solution? (reply: 10)
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buffer composition - (reply: 11)
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hepes solution storage - (reply: 4)
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strange western - transfer turned PVDF, filter, sponge, and buffer yellow? (reply: 6)
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(Alcian Blue) Staining Solution Preparation - (reply: 1)
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assay buffers - (reply: 1)
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Trouble growing bacteria in LB/amp solution - (reply: 5)
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Making a 80% ethanol/ 20% glacial acetic acid solution - (reply: 3)
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problems in concentration protein solution - (reply: 8)
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Guanidine-HCl solution storage - (reply: 1)
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DEPC before or after solution preparation?... - (reply: 4)
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Does this qualify as potassium phosphate buffer? - (reply: 3)
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for protein interaction what lysis buffer I should use? - (reply: 1)
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How to choose HindIII/EcoRI double digest buffer? - a stupid question, but I really curious about the answer (reply: 6)
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Agarose gels and SB Buffer - (reply: 7)
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How to make aurintricarboxylic acid (ATA) solution? - (reply: 1)
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question about phosphate buffer? - (reply: 1)
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Co-IP, nuclear proteins - buffers for lysis? - Who else does co-ip on nuclear proteins? (reply: 1)
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Phosphate Buffer - (reply: 5)
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Hank's balanced salt solution, do I need it? - Will PBS do instead? (reply: 3)
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Water or TE buffer for dissolving DNA - (reply: 5)
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good buffers - (reply: 1)
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concentration of SDS loading buffer ... - 1X buffer seems too little. (reply: 8)
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Inoue Methode - How to make the Buffer for the Inou eMethode (reply: 3)
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TAE BUFFER - (reply: 2)
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Ambion low conductivity electroporation buffer? - (reply: 1)
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buffer problems - (reply: 1)
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Potassium phosphate buffer - (reply: 2)
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Buffer for amonium sulphate precipitation - (reply: 1)
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Filtering solutions - (reply: 7)
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Lysis buffer with 1% tritonX - Kindly sugget me its other ingredients (reply: 1)
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Gel loading buffer - (reply: 12)
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help with buffer 5.1 for RNA extraction - (reply: 6)
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How to concentrate protein that already in SDS buffer and measure concentration? - Help please (reply: 4)
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Why do we use methanol in the transfer buffer? - Hope somebody can send me a reply in why do we use methanol in the transfer buff (reply: 3)
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Role of buffer in antibody purification - (reply: 3)
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Strange nanodrop readings after RNA isolation, strange solution - (reply: 13)
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Passive lysis buffer for luciferase assays - (reply: 11)
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to prepare westen blot transfer buffer, instead of methanol can i use ethanol. - (reply: 3)
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How to remove LPS from protein solution - (reply: 2)
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IP lysis buffer formulation : why adding a non-ionic detergent if a ? - (reply: 4)
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How to make malonate buffer? - (reply: 1)
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DNA, RNA and protein extraction using basic solution - (reply: 4)
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Why is TE frequently used as buffer for DNA solutions? - (reply: 1)
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PstI restrictase (Invitrogen) - reaction buffer (reply: 1)
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Annealing buffer for DNA oligos - RNA interference (reply: 2)
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Ampicillin - Problem in amipicillin solution prep. (reply: 13)
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STRIPPING BUFFER? - how to make good STRIPPING BUFFER? (reply: 3)
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50mM HEPES-KOH buffer pH7.4 formulation please - (reply: 1)
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Lysis Buffer Preparation - (reply: 3)
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FUNCTION OF BUFFER - (reply: 3)
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Emergent: Toxicity of cell lysis buffer :( - (reply: 2)
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8-MOP solution - (reply: 2)
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Role of restriction and TAE Buffer - (reply: 1)
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HEPES-KOH buffer - (reply: 5)
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NMDA buffer toxic? - (reply: 8)
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Change Tris for Phosphate buffer cause precipitation - Which buffer can replace Tris? (reply: 3)
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Stop Solution Doesn't Stop the Reaction - (reply: 3)
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buffer preparation for molecular biology - (reply: 9)
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what is the function of Na2EDTA in a buffer solution - (reply: 2)
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0.1M Phospahte Buffer, pH8.5 - Buffer Protocol (reply: 1)
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loading buffer concentration - (reply: 2)
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buffer exchange - (reply: 1)
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Storing of Tris/HCl buffer - (reply: 8)
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How to prepare potassium phosphate buffer - (reply: 6)
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orthovanadate in transfer buffer for WB? - immunodetection of phospho ERK1/2 (reply: 6)
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10% CHAPS solution (10ml) - Recipe wrong or right? (reply: 1)
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phosphate buffer confusion - the true way of preparation pls??!! (reply: 1)
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Making solutions for protein prep - (reply: 3)
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2-mercaptoethanol and TEMED solutions - (reply: 1)
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shelf life of PMSF/ leupeptin in buffer - (reply: 1)
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DNAse and NaF in lysis buffer...a good idea? - Does NaF or Na3VO4 inactivate DNAse I? (reply: 2)
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What is the role for gelatin in PCR buffer - (reply: 1)
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can i autoclave agarose gel with TAE buffer - (reply: 4)
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SDC in buffer? - (reply: 2)
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PPT in T4 DNA ligase buffer - (reply: 5)
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Lysis buffer for membrane protien - (reply: 1)
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What is the pH of 2x loading buffer? - (reply: 1)
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TIssue Homogenization Buffer W/o DTT - Missing Reagent (reply: 1)
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How to prepare primer solution - (reply: 7)
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Problem w/ ECL homemade solution for WB - Final solution works only for a few minutes! (reply: 7)
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Which buffers for storing of RNA and DNA? - (reply: 6)
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Is it ok to re-use Western Transfer Buffer - (reply: 4)
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Gel extraction and elution buffer - (reply: 4)
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Recipe for anti-fade solution?? - (reply: 1)
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His-taged protein is in 8M Urea, how to get rid of urea? - Ni-NTA column purified protein in 8M urea buffer (reply: 3)
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Help: western blot lysis buffer - (reply: 2)
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SDS-PAGE loading buffer - 1X, 2X, 4X loading buffer? (reply: 2)
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lysis buffer for western blot - lysis buffer (reply: 3)
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what these chemicals for in lysis buffer? - (reply: 3)
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About the maleic acid buffer - Maleic acid buffer can be autoclave? (reply: 1)
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Phosphate buffers - protein and NaCl affecting pH of buffer (reply: 4)
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preparation of solutions that need pH adjustment - (reply: 3)
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Balanced salt solutions - (reply: 2)
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How do you prepare PCR stock solution to minimize pipetting - (reply: 37)
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Plasmids in eluting buffer - (reply: 1)
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preparing buffer - (reply: 3)
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Azacytidine mother solution - (reply: 5)
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Buffer for red blood cell lysis for RNA extraction from blood - RNA methodology (reply: 6)
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Good buffer needed! - (reply: 7)
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How to prepare solution for drug or hormone treatment?? - Preparing dexamethasone and estradiol solution from powder (reply: 4)
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Difference between TE buffer at pH 8.0 and 7.5 - (reply: 2)
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Reuse TAE buffer and agarose gel? - yes or no... (reply: 22)
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Alginic acid - How to get it into solution? (reply: 4)
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Do enzymes work in different buffers? - (reply: 2)
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hydroproline assay citrate buffer - (reply: 1)
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Convert DNase units to weight - 2mg/ml from a 2U/ml solution (reply: 3)
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Preparation of Estrogen solution - (reply: 2)
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can RIPA buffer use to prepare nuclear protein? - (reply: 1)
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How to prepare the balance solution - (reply: 1)
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Lysis Buffer for MTT - (reply: 1)
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what solution to use for cell lysis - (reply: 5)
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endotoxin free buffers - (reply: 1)
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Buccal swab storage - is saline solution enough? (reply: 1)
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cell lysis - How do I decide what solution to use? (reply: 2)
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MES buffer in dialyzation - MES or any alternative for MES? (reply: 1)
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Why use imidazole as a buffer? - Imidazole Buffer (reply: 2)
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