Tris Tricine Gel Running Buffer - (Sep/27/2007 )
Hello. I was wondering if anyone has a recipe for the running buffer for a Tris Tricine gel where I can run both chambers in the same buffer. Our gel apparatus leaks and I think the anode and cathode buffers suggested by Schagger and VonJagow will mix. I know that some companies sell one buffer to run these gels in, does anyone know the components of this buffer? Thanks so much.
fix the leak.
if it is a gasket then add vacuum grease to seal the plate to the apparatus.
if it is the apparatus (ie-cracked plastic) then fuse it with methylene chloride.
run the gels the way they were intended to be run.
that being said, invitrogen sells novex tricine-sds running buffer.