denhardt's solution - (Jun/04/2007 )
anyone knows how to make denhardt's solution for hybridization after southern blot....without buying a kit?
here's the recipe for 100x Denhardt's solution:
10g Ficoll 400
+ 10g Polyvinylpyrrolidone
+ 10g Bovine Serum Albumin
fill up to 500 ml with H2O
by googling i got some recipies. we use powder from a kit of GE healthcare
Thank you very much!!!!