Protocol Online logo
Top : Forum Archives: : General Lab Techniques

denhardt's solution - (Jun/04/2007 )

anyone knows how to make denhardt's solution for hybridization after southern blot....without buying a kit?


Hi Solmaniar,

here's the recipe for 100x Denhardt's solution:

10g Ficoll 400
+ 10g Polyvinylpyrrolidone
+ 10g Bovine Serum Albumin
fill up to 500 ml with H2O



by googling i got some recipies. we use powder from a kit of GE healthcare

-T. reesei-

Thank you very much!!!!