MSP buffer composition - (Mar/07/2006 )
I was wondering about the usage of the special ammonium sulphate containing buffer for the methylation specific PCR mentioned by Herman et al.,1998 and in the MD Anderson methylation protocols. Does it go only with the ordinary Taq (used in Hot start MSP) or works equally well with enzymes like Amplitaq Gold/HotStar etc.,? I will be thankful from inputs from the methylation people.
We have used this buffer with a regular taq from sigma. It seems the buffer dosen't offer much advantage over buffer coming with the taq.