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transfer buffer - (Jul/21/2008 )

Has any one know how many times I can use my transfer buffer?
In second trial using my transfer buffer it doesnt work.Do you know what could be the reason?

-saraarasus-

QUOTE (saraarasus @ Jul 21 2008, 12:19 AM)
Has any one know how many times I can use my transfer buffer?
In second trial using my transfer buffer it doesnt work.Do you know what could be the reason?

i would not think of reusing the blotting buffer. it becomes very dirty after the run and would sabotage the subsequent transfer.

-rajgene-