Purpose of High ionic strength buffers - (Dec/02/2006 )
May i know what is the purpose/rationale of having high ionic strength buffers for Gel filtration chromatography (size exclusion chromatography)?
i think high molar buffers are used to exclude ions attached to gel beads for the purpose of elution..
thank you for your prompt reply. In other words, high molar buffers are for better elution of protein samples?once again, thank you:)
High ionic strength needed to avoid non-specific interactions with resin material