did NEBiolab change BamH1's buffer to buffer 3? - (Mar/26/2007 )
I ordered a BamH1 from NEB last week, but the buffer is NEBuffer3 , they converted it?? but the salt concentration for buffer 3 is only 100mM NaCl, compared with BamH1 buffer 150mM...............I've got star activity and smere from the cut of BamH1, anyone get any idea?
NEB buffer 2 works great for bam h1
I think there is too much enzyme. Reduce the amount of enzyme in use and the problem should go away.