SDS buffers - (Oct/16/2008 )
Hi every body;
i am new in SDS buffer preparation
i would like to prepare both the resolving & the stacking buffers &
i don't know which of them i will use, Tris base or Tris HCL
Tris HCL means Tris base +HCl.
HCl is used to adjust the pH of Tris base to pH8.8 for resolving buffer and pH6.8 for the stacking buffer.
Hope this may help.