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SDS buffers - (Oct/16/2008 )

Hi every body;

i am new in SDS buffer preparation

i would like to prepare both the resolving & the stacking buffers &
i don't know which of them i will use, Tris base or Tris HCL


Tris HCL means Tris base +HCl.
HCl is used to adjust the pH of Tris base to pH8.8 for resolving buffer and pH6.8 for the stacking buffer.

Hope this may help.

-Minnie Mouse-